市售肉苁蓉饮片的鉴别及苯乙醇苷类的含量测定

Determination of commercially available Cistanche pieces identification and phenylethanoid glycosides content

关键词:  鉴别  指纹图谱  质量评价

Key words:  HPLC  echinacoside  actcoside  quality evaluation

摘要:

[目的] 建立市售肉苁蓉饮片的高效液相色谱法（HPLC）指纹图谱，以期鉴别肉苁蓉真伪。建立HPLC方法测定其苯乙醇苷类含量。[方法] 采用HPLC法，以标准药材肉苁蓉为参照图谱，对肉苁蓉饮片进行指纹图谱分析。采用Kromasil C₁₈色谱柱，甲醇∶水（0.1%甲酸）梯度洗脱，检测波长330 nm。[结果] 该方法精密度、稳定性、重现性较好，不同市场采购的肉苁蓉指纹图谱差异较大。苯乙醇苷类含量测定方法符合要求。[结论] 福建、江苏和山东省购买的肉苁蓉饮片鉴定为伪品，其他省市购买的肉苁蓉饮片苯乙醇苷类含量符合要求，初步评价质量符合要求。

Abstract:

[Objective] Establish commercial Cistanche Pieces of HPLC fingerprint identification cistanche authenticity.Establish HPLC method for the determination of benzene in commercial cistanche glycosides content.[Methods] Standard Cistanche herbs as a reference fingerprint,using high performance liquid chromatography to analyze the fingerprint of Cistanche Pieces;Using Kromasil C₁₈ column,methanol:water (containing 0.1% formic acid) for gradient elution and wave length of detection was at 330 nm.[Results] The method precision,stability,good reproducibility,different market purchases of Cistanche fingerprints large differences.Phenylethanoid glycosides determination method meet the requirements.[Conclusion] Cistanche Pieces that purchase in Fujian,Jiangsu and Shandong province identified as counterfeit.Cistanche Pieces of the other provinces glycosides benzene content to purchase to meet the requirements,a preliminary evaluation of the quality to meet the requirements.