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| HPLC-ELSD测定心脑舒通胶囊中Terrestrinin A的含量 |
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张娜1,2, 栾晓莹1, 谢燕1,2, 柏自松1,2, 白洋1, 胡利民1, 常艳旭1, 王萌1, 张鹏1
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1.天津中医药大学, 天津市现代中药重点实验室, 天津 300193;2.天津国际生物医药联合研究院, 中药新药研发中心, 天津 300457
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| 摘要: |
| [目的] 建立心脑舒通胶囊及其总皂苷中Terrestrinin A含量的测定方法。[方法] 采用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）法，色谱柱为Hedra-C18柱（4.6 mm×150 mm，5 μm），流动相：乙腈-水，流速：1 mL/min，柱温：40℃，漂移管温度：60℃，气体（N2）压力：30 psi，增益值：100，进样量：20 μL。[结果] 线性范围：48.5~970.6 μg/mL，回归方程：Ŷ =1.6931X+1.2644（r2≥0.9990），精密度、重复性和稳定性实验的RSD值分别是1.21%、1.62%、1.90%，加样回收率是96.0%~103.2%，RSD值是2.40%。[结论] 该法简便可靠，可以应用于心脑舒通胶囊及其总皂苷中Terrestrinin A的含量测定及质量控制标准建立。 |
| 关键词: 心脑舒通胶囊 蒺藜 Terrestrinin A HPLC-ELSD 含量测定 |
| DOI：10.11656/j.issn.1673-9043.2017.02.14 |
| 分类号: |
| 基金项目:重大新药创制项目（2011ZX09201-201）；国家自然科学基金青年项目（81303183） |
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| Determination of Terrestrinin A from Xinnao Shutong capsules by HPLC-ELSD method |
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ZHANG Na1,2, LUAN Xiao-ying1, XIE Yan1,2, BAI Zi-song1,2, BAI Yang1, HU Li-min1, CHANG Yan-xu1, WANG Meng1, ZHANG Peng1
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1.Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China;2.Tianjin International Joint Academy of Biomedicine, Research and Development Center of Traditional Chinese Drug, Tianjin 300457, China
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| Abstract: |
| [Objective] To establish a quantitative method for the determination of Terrestrinin A in Xinnao Shutong capsules and total saponins. [Methods] Using HPLC ELSD method, chromatography column Hedra-C18 column (4.6 mm×150 mm, 5 microns), mobile phase:acetonitrile-water, flow rate:1 mL/min, column temperature:40℃, drift tube temperature:60℃, gas pressure (N2):30 psi, gain:100, sample quantity:20 μL. [Results] Linear range:48.5 to 970.6 μg/mL, the regression equation:Ŷ=1.693 1+1.264 4X (r2=0.9990), precision, repeatability and stability testing of RSD value was 1.21%, 1.62%, 1.9% respectively, the recovery rate is 96.0%-103.2%, RSD value was 2.4%. [Conclusion] The method is simple and reliable, and can be applied to determine the content of Terrestrinin A in Xinnao Shutong capsule and total saponins preserving method and establish quality control standards. |
| Key words: Xinnao Shutong capsules Tribulus terrestris L. Terrestrinin A HPLC-ELSD quantitative determination |
