桑葚多糖T3-3分离及秀丽隐杆线虫抗衰老活性研究

Optimization of the extracting parameters of mulberry polysaccharide and its anti-aging activity study

关键词:  桑葚多糖提取工艺  抗衰老活性  研究分析

Key words:  mulberry polysaccharide extacrion process  anti-aging activity  research and analysis

摘要:

[目的] 研究桑葚多糖T2提取工艺优化，并对其抗衰老活性进行分析。[方法] 以秀丽隐杆线虫为模型，用寿命、移动力分析、吞咽频率、产卵量和肠脂褐质综合评价不同剂量范围的桑葚多糖（T3-3）抗衰老活性。通过正交和优化实验确定最优提取工艺条件为：提取温度100℃，提取时间2 h，提取次数3次，料液比1：30，提取率最高达8.48%。[结果] 抗衰老活性研究表明，150 mg/L的T3-3能增强秀丽隐杆线虫的抗衰老能力。[结论] 该提取工艺1）延长了秀丽隐杆线虫的寿命，秀丽隐杆线虫平均寿命延长了10.38%（P<0.05），最大寿命延长了28.57%（P<0.05），50%存活率对应天数延长了0.91%。2）秀丽隐杆线虫吞咽频率增加，吞咽频率比对照组高18.78%。3）产卵量无统计学差异。4）减少秀丽隐杆线虫肠脂褐质的产生量，比对照组平均荧光强度低11.14%（P<0.05），实验末期，对照组的平均荧光强度增大了12.47%。

Abstract:

[Objective] To study the optimization of the extracting parameters of mulberry polysaccharide and its anti-aging activity. [Methods] Usingel egans model, such as life, movement analysis, frequency of swallowing, spawning and intestinal brown quality comprehensive evaluation of different dose of T3-3 to study the anti-aging activity of itspurified components T3-3. [Results] The best extracted condition was:100℃ of extraction temperate, 2 h of extraction time, 3 times, and 1:30 of liquid ratio, the extracted rate would up to 8.48%. Anti-aging activity:T3-3 extend the average life span for 10.38%(P<0.05), the corresponded time of 50% survival rate extend 0.91%; the deglutition frequency is 18% higher than the control group; the egg laying amount have no significant difference; the intestinal lipofusin produced by nematode is reduced, 11.14%(P<0.05) lower than the fluorescence intensity of control group. At the final stage of experiment, the average fluorescence intensity of control group increase 12.47%.[Conclusion] The results provide that the concentration of 150 mg/L, T3-3 has the best effect.