摘要: |
[目的] 建立不同批次黄芪指纹图谱的分析方法。[方法] 采用高效液相-蒸发光散射检测法(HPLC-ELSD )以Grace Apollo C18(250 mm×4.6 mm,5 μm)为色谱柱;流动相为乙腈和水,梯度洗脱,流速1.0 mL/min,蒸发光散射检测器,漂移管温度60℃,雾化器cooling,气压30psi (2.07 slpm),增益100。[结果] 建立的黄芪指纹图谱各色谱峰分离度较好,达到指纹图谱要求,相似度评价结果均大于0.96,证明不同批次间药材比较稳定。聚类分析法把12个批次的黄芪样品分为3类。建立了黄芪药材的HPLC-ELSD指纹图谱共有模型,找出了较大的17个共有峰。[结论] 为更好的控制黄芪的内在质量提供了可靠的分析方法。 |
关键词: 黄芪 指纹图谱 聚类分析 相似度评价 |
DOI:10.11656/j.issn.1673-9043.2017.02.16 |
分类号: |
基金项目:国家自然科学基金项目(81073033) |
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Establishment and analysis of HPLC-ELSD fingerprint of Radix Astragali |
YAN Zhi-xue, HE Yong-zhi, WANG Ying
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Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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Abstract: |
[Objective] To establish a fingerprint analysis method of Radix Astragali from various batches.[Methods] The fingerprint was performed by HPLC-ELSD. The conditions of chromatography:Grace Apollo C18(250 mm×4.6 mm,5 μm) was used with a mobile phase of CH3CN-H2O,flow rate:1.0 mL/min, tube:60℃, air pressure:30 psi(2.07 slpm), gain:100. [Results] The HPLC fingerprint for Astragali Radix was developed and 17 common peaks were determined from twelve different batches. The average similarity was over 0.96. The 12 samples were classified as 3 clusters by cluster analysis. [Conclusion] A reliable method was provided for controlling the quality of Radix Astragali. |
Key words: Radix Astragali fingerprint cluster analysis similarity evaluation |