屈膝点按叩揉法影响TGF-β/Smad通路促进损伤肌腱修复的作用机制研究

Mechanism research of Quxi dian爷an kourou method affact TG-茁/Smad pathway to promotes the repairation of damage tendon

关键词:  屈膝点按叩揉法  TGF-β/Smad通路  肌腱  修复

Key words:  Quxi dian'an kourou method  TGF-β/Smad pathway  tendon  repairation

摘要:

[目的]通过分析屈膝点按叩揉法对调控肌腱干细胞（TSCs）成肌腱分化相关通路蛋白的影响，探讨该手法对髌腱炎损伤修复的机制。[方法] 50只SD大鼠按随机数字表法随机分成5组，每组10只运用局部胶原酶Ⅰ注射建立大鼠髌腱炎模型，造模后治疗组采用屈膝点按叩揉法治疗，针剂组采用局部糖皮质激素注射治疗，CPM组运用持续被动运动（CPM）治疗仪治疗，模型组及正常组同条件饲养。通过实时荧光定量聚合酶链反应（qRT-PCR）和蛋白免疫印迹法（Western Blot）检测调控肌腱干细胞（TSCs）成肌腱分化相关通路蛋白的表达。[结果]治疗组、针剂组、CPM组转化生长因子β1（TGF-β1）、Smad-2、腱调蛋白（Tnmd）、胶原蛋白Ⅰ（CollagenⅠ）蛋白的表达均高于模型组，差异具有统计学意义（P<0.05），治疗组TGF-β1、Smad-2、Tnmd、CollagenⅠ蛋白的表达高于针剂组、CPM组，且差异有统计学意义（P<0.05）。[结论]屈膝点按叩揉法能够影响TGF-β/Smad信号通路调控TSCs分化行为产生对髌腱炎的修复作用。

Abstract:

[Objective] Through the analysis of Quxi dian'an kourou method affact the TSCs tendon differentiation by TGF-β/Smad pathway,finding out the mechanism of damage repairation.[Methods] Fifty SD rats were randomly divided into 5 groups,local injection of collagenase Ⅰ in rat patellar tendon to establish the model.After the model was established,we treat the treatment group with Quxi dian'an kourou method,injection group using the glucocorticoid,CPM group using the instrument of CPM,model group and the normal group were feeded in the same enviroment.Through the QRT-PCR and western blot method to detect the relative protain of TSCs tendon differentiation.[Results] Protein expression level of TGF-β1,Smad-2,Tnmd,CollagenⅠ in treatment group,injection group and CPM group higher than the model group (P<0.05).Protein expression level of TGF-β1,Smad-2,Tnmd,CollagenⅠ in treatment group higher than the injection group and CPM group (P<0.05).[Conclusion] Quxi dian'an kourou method can affact TGF beta/Smad pathway to control the behavior of TSCs tendon differentiation.