| 摘要: |
| [目的]利用超高效液相色谱串联四级杆飞行时间质谱技术（UPLC-Q-TOF-MS/MS）对血必净注射液中的化学成分进行快速鉴定。[方法]采用ACQUITY UPLC®BEH C18（2.1 mm×100 mm， 1.7 μm）色谱柱，以乙腈-0.1%甲酸水为流动相进行梯度洗脱，在负离子模式下进行数据采集，通过与对照品对比保留时间、相对分子量、二级质谱碎片离子的方法确定化学成分。[结果]在负离子模式下，45 min内鉴定出血必净注射液中20种化学成分。[结论]建立了一种简单、准确的UPLC-Q-TOF-MS/MS方法对血必净注射液中的化学成分进行鉴定。 |
| 关键词: 血必净注射液 UPLC-Q-TOF-MS/MS 定性分析 |
| DOI：10.11656/j.issn.1673-9043.2017.03.13 |
| 分类号:R284 |
| 基金项目:国家自然科学基金项目资助（81303140） |
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| Simultaneous identification of 20 components in Xuebijing injection by UPL——TO-MS/MS |
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TU Ya-ru1, OUYANG Hui-zi2, SUN Meng-jie1, CHANG Yan-xu1, HE Jun1
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1.Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To develop a UPLC-Q-TOF-MS/MS method for simultaneous identification of 20 components in Xuebijing injection.[Methods] The method of qualitative analysis was performed on ACQUITY UPLC®BEH C18(2.1 mm×100 mm,1.7 μm) column with a gradient mobile phase system of acetonitrile and 0.1% formic acid.All of the analytes were identified in negative ionization.According to the high resolution MS spectra data,fragmentation ion information andretention time,20 peaks were identified by comparison with reference standards data.[Results] Twenty components were identified in 45 min.[Conclusion] The established method for the identification of chemical constituents in Xuebijing injection is simple and accuracy. |
| Key words: Xuebijing injection UPLC-Q-TOF-MS/MS qualitative analysis |
