| 摘要: |
| [目的]建立同时测定玉女煎中芒果苷、β-蜕皮甾酮、毛蕊花糖苷含量的高效液相色谱法。[方法]采用Ultimate XB-C18色谱柱（4.6 mm×250 mm，5 μm）；以乙腈-0.1%磷酸溶液为流动相，梯度洗脱，流速：1.0 mL/min；检测波长：250 nm（芒果苷、β-蜕皮甾酮）和330 nm（毛蕊花糖苷）。[结果]芒果苷、β-蜕皮甾酮、毛蕊花糖苷质量浓度在10.0~400.0 μg/mL（r=0.999 9）、2.4~96 μg/mL（r=0.999 9）、2.0~80 μg/mL（r=0.999 9）范围内线性关系良好，3种成分的平均加样回收率（n=9）分别为100.2%、101.0%、98.1 8%，RSD分别为2.73%、1.95%、1.39%。[结论]该方法简便，重复性好，可为玉女煎的质量控制提供参考。 |
| 关键词: 玉女煎 梯度洗脱 同时测定 高效液相色谱法 芒果苷 β-蜕皮甾酮 毛蕊花糖苷 |
| DOI：10.11656/j.issn.1673-9043.2017.03.14 |
| 分类号:R284.2 |
| 基金项目:国家科技部“重大新药创制”专项课题（2013ZX09508-105） |
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| HPLC for simultaneous determination of various quota components in Yunvjian |
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WANG Ting-ting, WANG Ya-jing, SUN Ai-zhen, ZHOU Hao, ZHU Guang-mei, LI Na, ZHANG Zhe, TIAN Qian-yu
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Tianjin University of Traditional Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China
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| Abstract: |
| [Objection] To establish an HPLC method for simultaneous determina-tion of mangiferin,β-ecdysterone,verbascoside in Yunvjian.[Methods] The HPLC separetion was performed on Ultimate XB-C18 column (4.6 mm×250 mm,5 μm) eluted with the mobile phase as acetonitrile-0.1% phosporic acid solution in a gradient mode at a flow rate of 1.0 mL/min.The detection wavelength was 250 nm for mangiferin and β-ecdysterone,and 330 nm for verbascoside.[Results] The calibration curve was liner within the range of 10.0-400.0 μg/mL (r=0.999 9) for mangiferin,2.4~96.0 μg/mL (r=0.999 9) for β-ecdysterone,and 2.0~80.0 μg/mL (r=0.999 9) for verbascoside,and the average recovery were 100.2%,101.0%,98.1 8% with RSD were 2.73%,1.95% and 1.39%(n=9).[Conclusion] The method is simple and accurate,and can be used for the quality control of Yunvjian. |
| Key words: Yunvjian gradient elute simultaneoud determination HPLC mangiferin β-ecdysterone verbascoside |
