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| 参竹精片对大鼠心肌缺血/再灌注损伤的保护作用 |
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倪晶宇1,2,3, 王卫明1,2,3, 李澜1,2,3, 樊官伟4
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1.天津中医药大学, 天津市现代中药重点实验室——省部共建国家重点实验室培育基地, 天津 300193;2.方剂学教育部重点实验室, 天津 300193;3.天津市中药药理学重点实验室, 天津 300193;4.天津中医药大学第一附属医院, 天津 300193
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| 摘要: |
| [目的] 观察参竹精片对大鼠心肌缺血/再灌注损伤的保护作用。[方法] 选用健康雄性SD大鼠60只，随机分为模型组、参竹精片0.36、0.18、0.09 g/kg剂量组和维拉帕米对照组和假手术组，每组10只，各给药组按照设定的剂量灌胃，每日1次，连续7 d，假手术组和模型组给予等量的蒸馏水。给药7 d后，各组大鼠结扎冠状动脉左前降支缺血30 min、再灌注120 min，其中假手术组仅分离冠状动脉左前降支不结扎。观察大鼠心功能指标：左室射血分数（LVEF）、左心室短轴缩短率（LVFS）、左心室收缩末期容积（LV Vols）、左心室收缩末期内直径（LVIDs）、左心室舒张末期容积（LV Vold）、左心室舒张末期内直径（LVI Dd）；检测血清丙二醛（MDA）、超氧化物歧化酶（SOD）、乳酸脱氢酶（LDH）、肌酸激酶（CK）含量。[结果] 参竹精片0.18 g/kg剂量组可增加LVEF （P<0.05）、LVFS （P<0.01），降低了血清CK （P<0.01）；参竹精片0.36 g/kg剂量组降低了LDH （P<0.05）、MDA （P<0.01），升高了SOD （P<0.01）。[结论] 参竹精片可以减轻心肌缺血/再灌注过程中的损伤，对心功能具有一定保护作用。 |
| 关键词: 参竹精片 心肌缺血/再灌注损伤 抗氧化 |
| DOI：10.11656/j.issn.1673-9043.2017.04.11 |
| 分类号:R285.5 |
| 基金项目:教育部“创新团队发展计划”（IRT-16R54）。 |
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| The effects of protection of Shenzhujing tablet treatment on MIRI |
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NI Jing-yu1,2,3, WANG Wei-ming1,2,3, LI Lan1,2,3, FAN Guan-wei4
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1.Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China;2.Ministry of Education Key Laboratory of Formula of Traditional Chinese Medicine, Tianjin 300193, China;3.Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;4.The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
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| Abstract: |
| [Objective] To observe the effects of Shenzhujing tablet treatment on MI/R injury.[Methods] Sixty healthy SD rats were randomly divided into sham control group, model group, verapamil sheet group, and high, medium, low dose group of Shenzhujing tablet. After the pre-dosed 7 d, the rat model of myocardial ischemia/reperfusion was established by ligating the left anterior descending coronary ischemia 30 min, reperfusion 120 min. Cardiac function was evaluated using ultra-high resolution small animal imaging system, LVEF, LVFS, LVIDs, LV Vols, LVIDd, LV Vold were observed; serum MDA, SOD, lactate dehydrogenase (LDH), creatine kinase (CK) were detected.[Results] Shenzhujing tablet 0.18 g/kg may significantly improve ejection fraction (P <0.05), left ventricular shortening fraction (P<0.01),and reducethe level of serum CK (P<0.01); Shenzhujing tablet 0.36 g/kg can reduce the level of serum LDH(P<0.05), MDA(P<0.01), and increase the level of serum SOD(P<0.01).[Conclusion] Shenzhujing tablet can reduce myocardial ischemia/reperfusion damage and improve the cardiac function. |
| Key words: Shenzhujing tablet myocardial ischemia/reperfusion injury anti-oxidation |
