| 摘要: |
| [目的] 观察氧化修饰的低密度脂蛋白（ox-LDL）对体外培养的血管内皮细胞（ECV-304）CD40信号的影响及黄芪甲苷的抗炎作用。[方法] 100 mg/L ox-LDL刺激ECV-304细胞24 h后，以实时定量聚合酶链式反应（Realtime PCR）和蛋白免疫印迹法（Western blot）测定细胞CD40 mRNA和蛋白的表达，酶联免疫吸附（ELISA）法测定细胞上清液肿瘤坏死因子-α （TNF-α）、可溶性血管细胞间黏附因子-1（sVCAM-1）、白介素-6（IL-6）和白介素-8（IL-8）的含量。以拮抗性抗CD40抗体，抑制CD40的作用后，再次观察上清液TNF-α、sVCAM-1、IL-6和IL-8的含量变化。20μg/mL的黄芪甲苷预孵育4 h，再以100 mg/L ox-LDL刺激ECV-30424 h，测定细胞CD40 mRNA、CD40蛋白的表达及上清液TNF-α、sVCAM-1、IL-6和IL-8的含量。[结果] 1）ox-LDL上调细胞CD40 mRNA和CD40蛋白的表达。2）ox-LDL促进细胞分泌TNF-α、sVCAM-1、IL-6和IL-8；而抑制CD40蛋白则抑制细胞分泌TNF-α、sV-CAM-1、IL-6和IL-8。3）黄芪甲苷下调CD40 mRNA、CD40蛋白表达，抑制内皮细胞分泌TNF-α、sVCAM-1、IL-6和IL-8。[结论] 黄芪甲苷通过抑制CD40信号下调ox-LDL所致内皮细胞炎症介质的表达，是其抗动脉粥样硬化的潜在机制。 |
| 关键词: 内皮细胞 氧化修饰的低密度脂蛋白 CD40 抗炎症 动脉粥样硬化 |
| DOI：10.11656/j.issn.1673-9043.2017.04.12 |
| 分类号:R285.5 |
| 基金项目:国家自然科学基金项目（81573733）。 |
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| Effect of the expression of CD40 signal in endothelial cells with ox-LDL and anti-inflammatory effect of Astragaloside IV |
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WEI Bing, JIANG Xi-juan, LU Bin, SUN Ying-xin, LI Hu-hu, ZHANG Tong, LI You-you, GUO Mao-juan
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Tianjin University of Traditional Chinese Medcine, Tianjin 300193, China
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| Abstract: |
| [Objective] Effect of the expression of CD40 signaling in endothelial cells (ECV-304) with modified low density lipoprotein (ox-LDL) and anti-inflammatory effects of Asragaloside IV.[Methods] ECV-304 with 100 mg/L ox-LDL for 24 h, then measured the expression of CD40 mRNA and protein by Real-time PCR and Western blot. The content of TNF, sVCAM-1, IL-6 and IL-8 in cell supernatant were determined by ELISA assay. With anti-CD40 antibody was added to ECV-304, and so did the same of TNF-α, sVCAM-1, IL-6 and IL-8 in cell supernatant. ECV- 304 with 20 g/mL Astragaloside IV preincubation for 4 h, and then with 100 mg/L ox-LDL for another 24 h, checking the CD40 mRNA and protein and the content of TNF-α, sVCAM-1, IL-6 and IL-8 in cell supernatant.[Results] The expression of CD40 mRNA protein were up-regulated with Ox-LDL in ECV-304, and promoted cell secretion of TNF-α, sVCAM-1, IL-6 and IL-8.However, the protein of CD40 were inhibited, and TNF-α, sVCAM-1, IL-6 and IL-8 were all down- regulated. Astragaloside IV could down regulate the expression of CD40 mRNA and protein, and inhibit the secretion of TNF-α, sVCAM-1, IL-6 and IL-8.[Conclusion] Astragaloside IV could inhibit the expression of ox-LDL induced inflammatory mediators in endothelial cells by inhibiting CD40 signaling, which is a potential mechanism of anti-atherosclerosis. |
| Key words: endothelial cells ox-LDL CD40 anti-Inflammatory atherosclerosis |
