复方血脂宁中4种活性成分的降解动力学研究

Degradation kinetics of four active ingredients in compound of Xuezhining

关键词:  复方血脂宁  pH  温度  降解动力学

Key words:  compound of Xuezhining  pH  temperature  degradation kinetics

摘要:

[目的]研究复方血脂宁中4种主要活性成分在不同pH和温度条件下的降解动力学规律。[方法]建立高效液相色谱分析方法，考察不同pH（1.2、6.8、8.3）、不同温度（60、70、80、90℃）条件下血脂宁中4种活性成分单体：红镰霉素-6-O-β-D-龙胆二糖苷（红镰霉素）、二苯乙烯苷、荷叶碱、金丝桃苷的稳定性，并利用化学动力学方法计算降解反应动力学参数。[结果]红镰霉素、二苯乙烯苷、荷叶碱和金丝桃苷在不同pH和温度条件下均发生不同程度的降解，且均符合一级动力学规律；温度对降解速率影响较大，温度升高降解速率明显加快。根据Arrhenius方程计算得到，红镰霉素、二苯乙烯苷、荷叶碱和金丝桃苷4种成分单体在水溶液中的降解活化能分别为90.56、74.66、72.66、74.42 kJ/mol。[结论]复方血脂宁中4种活性成分在高温条件下均易降解。室温条件下，在不同pH值缓冲液中，荷叶碱和红镰霉素降解较慢，而金丝桃苷、二苯乙烯苷在弱碱性条件下降解较快。

Abstract:

[Objective] To study the degradation kinetics of four main active ingredients in compound of Xuezhining under different pH values and temperatures.[Method] HPLC method was established to study the stability ofRubrofusarin-6-O-β-gentiobioside(HLMS), 2, 3, 5, 4-tetrahy-droxystilbene-2-O-β-D-glycoside(THSG), Nuciferine (HYJ) and Hyperoside (JSTG),four active ingredients in compound of Xuezhining, under different pH values (1.2, 6.8 and 8.3) and different temperatures (60, 70, 80, 90℃). Degradation kinetic parameters were evaluated by chemical kinetics.[Results] The results indicated that HLMS, THSG, HYJ and JSTG were degraded at different degree under different pH values and temperatures, and accord with the first-order kinetic. Temperature has great effect on the degradation rate, and the degradation rate increased significantly with the increase of temperature. According to arrhenius equation, activation energy (Ea) of HLMS, THSG, HYJ and JSTG in aqueous solution were 90.56 kJ/mol,74.66 kJ/mol, 72.66 kJ/mol and 74.42 kJ/mol, respectively.[Conclusion] The four active ingredients in compound of Xuezhining were sensitive to high temperature condition. At the condition of room temperature, HYJ and HLMS were slower in different pH buffer solution, while JSTG and THSG degraded faster in weak alkalinity condition.