| 摘要: |
| [目的]研究荷叶中的苷类化学成分，为荷叶化学成分的系统研究、质量控制及新药开发提供参考依据。[方法]综合利用硅胶、十八烷基键合硅胶（ODS）、凝胶（Sephadex LH-20）等柱色谱以及制备高效液相色谱（pHPLC）进行分离纯化，最后通过质谱（MS）、核磁（NMR）以及与已知文献比对等方法，鉴定各化合物的结构。[结果]荷叶经70%的乙醇提取，通过各种分离纯化方法，共得到9个单体化合物，分别为：异槲皮苷（1）、槲皮素-3-O-β-D-吡喃木糖-（1→2）-β-D-吡喃半乳糖苷（2）、槲皮素-3-O-β-D-葡萄糖醛酸-6"-甲酯（3）、槲皮素-3-O-β-D-葡萄糖醛酸-6"-乙酯（4）、山奈酚-3-O-β-D-吡喃半乳糖苷（5）、鼠李素-3-O-β-D-吡喃葡萄糖苷（6）、柯伊利素-7-O-β-D-吡喃葡萄糖苷（7）、（+）-松脂醇-4-O-β-D-吡喃葡萄糖苷（8）、（+）-表松脂醇-4'-O-β-D-吡喃葡萄糖苷（9）。[结论]3-4，8-9为莲属植物中首次分离得到的化合物。 |
| 关键词: 荷叶 苷类成分 分离纯化 结构鉴定 |
| DOI：10.11656/j.issn.1673-9043.2017.05.13 |
| 分类号:R284.2 |
| 基金项目:重大新药创制专项-中成药药性优化技术研究（2015ZX09501004-003-004）。 |
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| Isolation and identification of glycosides from the leaves of Nelumbo nucifera Gaertn. |
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WANG Hao1, XIA Ming-hui2, PANG Xu3, HAN Li-feng3, ZHANG Yan-jun4
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1.College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Cangzhou central hospital, Cangzhou, Tianjin 061000, China;3.Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;4.Office of Academic Affairs, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To investigate the glycosides from the lotus leaf in order to further research on quality control and new drug development.[Methods] The glycosides were separated by silica gel, ODS, Sephadex LH-20 column chromatographies, as well as preparative HPLC, the structures were identified by MS, NMR spectra and comparison with literatures.[Results] Nine compounds were purified from 70% ethanol extract of lotus leaves and identified as isoquercitrin (1), quercetin-3-O-β-D-xylopyranosyl(1→2)-β-D-galactopyranoside (2), quercetin-3-O-β-D-glucuronide-6"-methyl ester (3), quercetin-3-O-β-D-glucuronide-6"-ethyl ester (4), kaempferol 3-O-β-D-galactopyranoside (5), chrysoeriol-7-O-β-D-glucopyranoside (6), chrysoeriol-7-O-β-D-glucopyranoside (7), (+)-pinoresinol-4-O-β-D-glucoside (8), (+)-epipinoresinol-4'-O-β-D-glucoside (9).[Conclusion] Compounds 3, 4, 8 and 9 were obtained from genus of Nelumbo for the first time. |
| Key words: Nelumbinis Folium glycosides chemical constituents structure identification |
