合煎与单煎对四物汤中4种化学成分溶出量的影响

Effect of four chemical components dissolution in Siwu decoction by decocting together and single

关键词:  四物汤  合煎  单煎  芍药苷  阿魏酸  藁本内酯  洋川芎内酯I

Key words:  Siwu decoction  mixed-decoction  single-decoction  peoniflorin  ferulic acid  ligustilide  senkyunolide I

摘要:

[目的]建立同时测定四物汤水提液中芍药苷、阿魏酸、藁本内酯和洋川芎内酯I 4种化学成分含量测定的方法，并考察合煎与单煎对这4种成分溶出量的影响。[方法]采用反相高效液相色谱法，色谱柱为Cosmosil C₁₈（4.6 mm×250 mm，5 μm），流动相为甲醇-0.3%甲酸水溶液梯度洗脱，检测波长为280 nm，流速为1 mL/min，柱温为30℃，分析在合煎、单煎条件下四物汤中4种化学成分溶出量的变化。[结果]芍药苷、阿魏酸、藁本内酯、洋川芎内酯I在19.38~310.00 μg/mL（r=0.999 4），1.51~24.16 μg/mL（r=0.999 4），1.50~24.00 μg/mL（r=0.999 6），2.00~32.00 μg/mL（r=0.999 4）线性范围内关系良好；平均加样回收率分别为95.89%（RSD=2.90%），97.08%（RSD=2.28%），96.84%（RSD=2.87%），98.77%（RSD=1.98%）。四物汤合煎液中洋川芎内酯I的溶出量低于当归、川芎药材溶出总量，而阿魏酸和藁本内酯的溶出量均高于当归、川芎药材溶出总量，芍药苷溶出量高于芍药药材溶出量。[结论]合煎与单煎中化学成分的溶出量并不是组方药味的简单叠加，而有其内在的配伍规律。

Abstract:

[Objective] To establish a method for content determination of peoniflorin, ferulic acid, ligustilide and senkyunolide I in Siwu decoction and study the dissolution variation between mixed-decoction and single-decoction.[Methods] RP-HPLC with Cosmosil C₁₈ (4.6 mm×250 mm, 5 μm) was used, methanol-0.3% formic acid solution gradient elution was applied. The flow rate was 1.0 mL/min. The detection wavelength was 280 nm and the column temperature was 30℃. The dissolution variation was analyzed between mixed-decoction and single-decoction.[Results] The linear range of four components were 19.38~310.00 μg/mL (r=0.999 4), 1.51~24.16 μg/mL (r=0.999 4), 1.50~24.00 μg/mL (r=0.999 6) and 2.00~32.00 μg/mL (r=0.999 4) with average recovery 95.89%(RSD=2.90%), 97.08%(RSD=2.28%), 96.84%(RSD=2.87%) and 98.77%(RSD=1.98%) respectively. The dissolution of senkyunolide I was lower mixed-decoction than single-decoction of Angelica and Chuanxiong. The dissolution of ferulic acid and ligustilide were higher mixed-decoction than single-decoction of Angelica and Chuanxiong. The dissolution of peoniflorin was higher mixed-decoction than single-decoction of Radix paeoniae Alba.[Conclusion] The dissolution of chemical compositions were not a simple superposition of their dissolution in single medicine, but have their own inherent compatibility regularity. This research from the perspective of the chemical composition dissolution provides a reference for the study of Siwu decoction.