| 摘要: |
| [目的]探究复方颠倒凝胶对大鼠耳廓痤疮模型的治疗作用。[方法]大鼠右耳廓涂抹油酸制造大鼠耳廓痤疮模型，连续涂抹21 d。造模成功后，将大鼠随机分为正常对照组、生理盐水组、阿达帕林凝胶组和复方颠倒凝胶低、中、高剂量组，分别对比用药14 d。而后切取大鼠右耳廓，进行肉眼和镜下观察。根据判定分级标准对实验结果进行分析，探究复方颠倒凝胶的抗痤疮作用。[结果]造模成功后，大鼠右耳廓皮肤粗糙，耳廓明显增厚变硬，皮肤红肿伴皮温升高，镜下组织学观察病理改变明显。用药治疗后阿达帕林凝胶组和复方颠倒凝胶高剂量组大鼠耳廓痤疮症状均明显减轻，镜下观察接近正常组织，复方颠倒凝胶低、中剂量组较高剂量组恢复率较低。阿达帕林凝胶组和复方颠倒凝胶各组大鼠恢复情况均明显优于生理盐水组（P<0.001），而复方颠倒凝胶低剂量组和高剂量组之间差异有统计学意义（P<0.05）。[结论]复方颠倒凝胶对大鼠耳廓痤疮模型具有较好的治疗作用，且存在一定的量效关系。 |
| 关键词: 复方颠倒凝胶 阿达帕林凝胶 大鼠耳廓痤疮模型 抗痤疮作用 量效关系 |
| DOI：10.11656/j.issn.1673-9043.2017.05.18 |
| 分类号:R285.5 |
| 基金项目:天津市市级大学生创新创业训练计划项目（201610063021）；武警后勤学院附属医院科研平台开放基金（WYKFM200609）。 |
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| Study on therapeutic effect of compound Diandao gel on rat auricle acne model |
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LIN Shan-shan1, JIN Xin2, HUANG Wei3, LI Pei-chun3, LIU Rui3, NIAN Li1
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1.College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Department of Pharmacology, Logistics University of Chinese People's Armed Police Force, Tianjin 300309, China;3.School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To explore the therapeutic effect of compound Diandao gel on the rat auricle acne model.[Methods] The rat auricle acne model was made by applying oleic acid to the right auricle of rat for 21 days. After model was established, all rats were divided into normal control group, saline group, adapalene gel group and low-dose, medium-dose, high-dose compound Diandao gel group. These rats were treated with different drugs for 14 days. Then the right auricle of rats was cut out and observed by the naked eye and microscopy. The experimental results were evaluated according to the grading standard for exploring the anti-acne effect of compound Diandao gel.[Results] After the model was established successfully, the skin of the right auricle of the rats was rough and thickened and hardened significantly. The skin was red and swollen, and the skin temperature was elevated. Histopathological observation by microscopy showed obvious pathological changes. After rats were treated with different drugs, the symptoms of auricle acne of rats in adapalene gel group and high-dose compound Diandao gel group were obviously alleviated, and the histological observation showed that auricles were close to normal tissue. The recovery rate of the low-dose group and middle-dose group was lower than that of the high-dose group. The recovery of the adapalene group and every compound Diandao gel groups was significantly better than that of the saline group (P<0.001). However, there was significant difference between low-dose and high-dose compound Diandao group (P<0.05). There was no significant difference between adapalene gel group and medium-dose, high-dose compound Diandao gel group (P>0.05).[Conclusion] Compound Diandao gel on the rat auricle acne model has a good therapeutic effect, and there is a certain dose-effect relationship. |
| Key words: compound Diandao gel adapalene gel rat auricle acne model anti-acne effect dose-effect relationship |
