血必净注射液及其药代标示物对脓毒症大鼠炎性因子TNF-α、IL-1、IL-6、IL-8和IL-10表达的影响

Effect of treatment with Xuebijing injection and its pharmacokinetics markers on serum inflammatory factor indude TNF-α, IL-1, IL-6, IL-8 and IL-10 in rats with sepsis

关键词:  血必净注射液  药代标示物  脓毒症  炎性因子

Key words:  Xuebijing injection  pharmacokinetics markers  sepsis  inflammatory factor

摘要:

[目的]研究血必净注射液及其药代标示物对脓毒症大鼠炎性因子肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和白细胞介素-10（IL-10）表达的影响。[方法] 30只雄性SD大鼠随机分为空白组、模型组、血必净组、药代标示物组、左氧氟沙星组（阳性对照组），静脉注射给药后采用盲肠结扎穿孔术（CLP）制备脓毒症大鼠模型，并于给药后5 h于大鼠眼眦静脉取血，离心取上清液。将取得的上清液采用酶联免疫吸附法（ELISA）分别检测TNF-α、IL-1、IL-6、IL-8和IL-10的含量。[结果]血必净组、左氧氟沙星组和药代标示物组在给药5 h后，脓毒症模型大鼠血清中炎性因子TNF-α、IL-1和IL-8有显著降低。[结论]血必净注射液以及其药代标示物能够显著抑制因炎症刺激造成的炎性因子的过度分泌。

Abstract:

[Objective] To study the effect of treatment with Xuebijing injection and its pharmacokinetics markers on serum inflammatory factor, indude serum tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), in rats with sepsis.[Methods] Rats were divided into five groups (each 6 rats, n=6) i.e. blank operation, sepsis, sepsis with Xuebijing treatment, Xuebijing pharmacokinetics markers treatment, and levofloxacin treatment group (positive control group). After intravenous injection, sepsis was reproduced in rats by cecal ligation and puncture. Blood samples were collected at 5 h after administration via fossa orbitalis. The concentration of TNF-α, IL-1, IL-6, IL-8 and IL-10 was determined by enzyme linked immunoad sordent assay (ELISA).[Results] The study shows that the concentration of TNF-α, IL-1 and IL-8 were significantly increased in the group of sepsis with Xuebijing treatment, Xuebijing pharmacokinetics markers treatment, and levofloxacin treatment.[Conclusion] Xuebijing injection and its pharmacokinetics markers can inhibit the release of serum inflammatory factorin rats with sepsis.