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不同采收期和部位黔产千里光中总黄酮、绿原酸和金丝桃苷含量动态比较
李燕, 韩忠耀, 魏学军
贵州省黔南民族医学高等专科学校, 都匀 558000
摘要:
[目的]研究不同采收期及部位黔产千里光中总黄酮、绿原酸和金丝桃苷含量的动态变化规律,结合生物产量,为确定黔产千里光的采收期和药用部位提供科学理论依据。[方法]采用紫外分光光度法测定总黄酮,高效液相色谱法测定绿原酸和金丝桃苷的含量。[结果]不同采收期黔产千里光总黄酮的含量以6月份含量最高,绿原酸和金丝桃苷含量均以8月份最高,不同部位千里光叶中总黄酮、绿原酸和金丝桃苷的含量最高;8月份千里光的生物产量最大,茎、叶的产量占较大比重。[结论]综合考虑3种活性成分的含量和生物产量,建议黔产千里光在7-9月份采收地上部位为宜。
关键词:  千里光  总黄酮  绿原酸  金丝桃苷  含量测定  紫外分光光度法  高效液相法  生物产量
DOI:10.11656/j.issn.1673-9043.2018.02.16
分类号:R284.2
基金项目:贵州省教育厅自然科学研究资金项目(20090119);黔南民族医学高等专科学校资金项目(QNYZ201410)。
Dynamic comparison of the content of total Flavones,Chlorogenic Acid and Hyperoside in various parts of GuiZhou Senecio scandens Buch. -Ham. in Different Seasons
LI Yan, HAN Zongyao, WEI Xuejun
Qiannan Medical College for Nationalities, Duyun 558000, China
Abstract:
Objective:To compare the content of total flavones, chlorogenic acid and hyperoside in GuiZhou Senecio scandens Buch.-Ham. in different seasons and different parts,and combine with biological yield to provide a basis for determining the harvest times and parts of GuiZhou Senecio scandens Buch.-Ham.[Methods] The content of total flavones was determined by UV,The content of chlorogenic acid and hyperoside were determined by HPLC.[Result] The content of total flavones that was extracted in June was highest. The content of chlorogenic acid and hyperoside were highest in August. The content of total flavones,chlorogenic acid and hyperside in leaves was much higher than in the roots and the stems.The biological yield in August was highest. The stem and leaf biological yield for a large proportion, and the root yield was the lowest.[Conclusion] Considering content of total flavonoids, chlorogenic acid, hyperoside and biological yield, It is advisable to harvest the aerial parts of Sen.ecio scanden.s Buch.-Ham in July,August and september.
Key words:  Senecio scandens Buch.-Ham.  Total flavones  Chlorogenic acid  Hyperoside  contents determination  UV  HPLC  biological yield
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