参麦注射液协同奥沙利铂抗结肠肿瘤作用

Effect of Shenmai injection combined with oxaliplatin on colon cancer

关键词:  参麦注射液  奥沙利铂  结肠癌  血管生成

Key words:  Shenmai injection  oxaliplatin  colon cancer  angiogenesis

摘要:

[目的]通过观察参麦注射液联合奥沙利铂对结肠癌LOVO细胞裸鼠移植瘤的影响探究参麦注射液与奥沙利铂合用增效减毒的作用机制。[方法]制备裸鼠LOVO细胞移植瘤体内模型，设立生理盐水组（n=8）、奥沙利铂组（8 mg/kg，n=8）、联合治疗组（奥沙利铂8 mg/kg；参麦注射液10 mg/g，n=8）及参麦组（参麦注射液10 mg/g，n=8），通过腹腔注射途径给药，自给药之日起每隔1日记录肿瘤体积并绘制生长曲线。给药14 d后观察奥沙利铂对移植瘤体抑瘤率的影响：处死小鼠后留取血样；摘取脾组织称质量以计算脾指数；摘取肿瘤组织并应用免疫组织化学法检测肿瘤组织中碱性成纤维细胞生长因子（b-FGF）及血小板-内皮细胞黏附分子（CD31）的表达。[结果]参麦注射液与奥沙利铂联合用药抑瘤率为49%，联合用药组抑制肿瘤生长的作用显著且对荷瘤鼠血液及肝肾无明显毒性；联合用药组可显著降低b-FGF的表达，并降低肿瘤组织的微血管密度（MVD）。[结论]参麦注射液能减轻奥沙利铂对小鼠免疫器官与肝功能的影响，并增强奥沙利铂抗结肠恶性肿瘤的作用，其机制可能与抑制b-FGF介导的血管生成信号通路相关。

Abstract:

[Objective] To study the mechanism of synergistic attenuating effect of Shenmai injection combined with oxaliplatin by observing the effect of Shenmai injection combined with oxaliplatin on transplanted tumors of colon cancer LOVO cells in nude mice.[Methods] An in vivo model of nude mice with LOVO cell transplantation was established. NC group, oxaliplatin group (8 mg/kg), combination group (oxaliplatin 8 mg/kg; Shenmai injection group (10 mg/g) and Shenmai injection group(Shenmai injection 10 mg/g) were set up. Administered by intraperitoneal injection. The tumor volume was recorded every other day from the date of administration. The growth curve was drawn. The effect of oxaliplatin on the tumor inhibition rate of the transplanted tumor was observed after 14 d of administration. Blood samples were taken after the mice were sacrificed, the spleen tissue was weighed to calculate the spleen index; the tumor tissue was removed. The expression of basic fibroblast growth factor (b-FGF) and platelet-endothelial cell adhesion molecule (CD31) in tumor tissue was detected by chemical method.[Results] The tumor inhibition rate of combination of Shenmai injection and oxaliplatin was 49%. Combination therapy could inhibit the growth of tumor tissues and had no obvious toxicity to the liver and kidney function of tumor-bearing mice. The combined treatment group could significantly reduce the expression of b-FGF and reduce the microvessel density (MVD) of tumor tissue.[Conclusion] Shenmai injection can reduce the effect of oxaliplatin on immune organs and liver function in mice. The mechanism of enhancing anti-colon cancer Oxaliplatin action may be related to the inhibition of angiogenesis signaling pathways mediated by b-FGF.