延胡索炮制前后多组分质量控制方法的研究

Study on the method of multi-component quality control before and after processing of corydalis yanhusuo

关键词:  延胡索  UPLC  生物碱  含量  炮制

Key words:  Corydalis yanhusuo  UPLC  alkaloid  content  process

摘要:

[目的] 建立超高效液相色谱法（UPLC）同时测定延胡索药材中四氢非洲防己碱、原阿片碱、四氢黄连碱、非洲防己碱、延胡索乙素、盐酸小檗碱、盐酸巴马汀、去氢紫堇碱8种生物碱含量的方法，并对延胡索生品及炮制品进行测定。[方法] 采用UPLC检测方法，ACQUITY UPLC BEH C₁₈色谱柱（100 mm×2.1 mm，1.7 μm），乙腈（B）-0.2%甲酸水溶液，梯度洗脱，流速：0.3 mL/min，柱温55℃，检测波长280 nm。[结果] 四氢非洲防己碱，原阿片碱、四氢黄连碱、非洲防己碱、延胡索乙素、盐酸小檗碱、盐酸巴马汀、去氢紫堇碱在0.395～98.8、0.4～100.2、0.99～247.5、0.197～49.4、1.004～251、0.198～49.5、0.398～99.6、1～250 μg/mL浓度范围内线性良好，平均加样回收率在98.10%～100.87%，RSD≤1.94（n=6）。[结论] UPLC所建立的方法简便、快速、准确，可应用于延胡索药材中多种成分含量测定，为延胡索药材质量控制提供更加科学、便捷的方法。

Abstract:

[Objective] To establish an UPLC method for the simultaneous determination of eightalkaloids(tetrahydrocolumbamine,protopine,tetrahydrocoptisine,columbamine,tetrahy-dropalmatine,berberine hydrochloride,palmatine hydrochloride and dehydrocorydaline) in corydalis yanhusuofrom the products and processed products.[Methods] The separationwas performed on an ACQUITY UPLCBEH C₁₈ (100 mm×2.1 mm,1.7 μm) columnin the UPLC method. The mobile phases consisted of acetonitrile (B) and 0.2% formic acid aqueous solutionwith gradient elution. The flow rate was 0.3 mL/min,the column temperature was 55℃,and the detection wavelength was 280 nm.[Results] Good linearities oftetrahydrocolumbamine,protopine,tetrahydrocoptisine,columbamine,tetrahydropalmatine,berberine hydrochloride,palmatine hydrochloride and dehydrocorydaline were achievedwithin the range of 0.395~98.8、0.4~100.2、0.99~247.5、0.197~49.4、1.004~251、0.198~49.5、0.398~99.6、1~250 μg/mL,respectively. The average recoveries were in the range of 98.10%-100.87%,RSD ≤ 1.94 (n=6).[Conclusion] The method established by UPLC is simple,rapid and accurate,and it can be applied to the determination of various components in Corydalis yanhusuo,and provide a more scientific and convenient method for the quality control of Corydalis yanhusuo.