姜黄素对结肠炎小鼠结肠组织相关细胞因子表达的调控作用

Regulatory effect of curcumin on expression to relevant cytokines in colonic tissue about colitis mice

关键词:  姜黄素  结肠炎  细胞因子  作用机制

Key words:  curcumin  colitis  cytokines  mechanism

摘要:

[目的] 探讨不同剂量姜黄素对结肠炎（UC）小鼠的疗效和作用机制。[方法] 把小鼠分成正常组、模型组、姜黄素低、中、高剂量组、美沙拉嗪组，采用三硝基苯磺酸（TNBS）复制小鼠结肠炎模型后，经姜黄素治疗7 d，观察小鼠体重和粪便性状等一般生活习性；分离结肠进行HE染色观察组织病理学形态改变及评分以进行疗效评价；ELISA检测相关细胞因子水平。[结果] 模型组与正常组比较，结肠组织上清液中GM-CSF、IL-2、IL-12P40、IL-21的表达上升，而IL-4、IL-15和IL-23的量则下降。经姜黄素治疗后的小鼠与模型组相比，结肠组织上清液中GM-CSF、IL-2、IL-12P40、IL-21的表达降低，IL-4、IL-15和IL-23的量升高。[结论] 姜黄素可通过抑制促炎因子的表达，同时增加抑炎因子分泌而有效治疗结肠炎。

Abstract:

[Objective] To investigate the curative effect and possible mechanism of curcumin in different colon effect on colitis in mice.[Methods] The C57 mice randomly were divided into six groups,including the normal model group(saline),the model group(saline),curcumin low(50 mg/kg),middle(100 mg/kg) and high(200 mg/kg) dose groups,mesalazine group(300 mg/kg). After trinitrobenzene sulfonic acid (TNBS) was used to replicate the mouse colitis model,the mice were treated by 7 days to observe the general living habits of the rats such as weight,activity,hair gloss and fecal characters,the colonic mucosal injury score was observed and the pathological changes of the colon were observed by HE staining and the score was evaluated for curative effect. The level of GM-CSF,IL-2,IL-4,IL-12P40,IL-15,IL-21 and IL-23 were detected by ELISA.[Results] In the model group the levels of GM-CSF,IL-2,IL-12P40 and IL-21 in colonic supernatants increased,while the levels of IL-4,IL-15 and IL-23 decreased. Compared with untreated TNBS-induced colitis mice,the curcumin could reduce the expression of IL-2,IL-12P40 and IL-21 in the supernatant of colonic tissue of rats,and increase the levels of IL-4,IL-15 and IL-23.[Conclusion] The curcumin has therapeutic effect on colitis,and its possible mechanism is to regulate the cytokine balance and reduce inflammatory injury.