基于网络药理学的理气化痰活血方治疗心脏X综合征分子机制研究

Study on the mechanism of qi-regulating,phlegm-resolving,and blood-promoting prescription in treating cardiac syndrome X Based on network pharmacology

关键词:  网络药理学  理气化痰活血方  心脏X综合征  信号通路

Key words:  network pharmacology  qi-regulating,phlegm-resolving,and blood-promoting prescription  cardiac syndrome X  signaling pathway

摘要:

[目的] 运用网络药理学分析理气化痰活血方治疗心脏X综合征的分子机制。[方法] 从TCMSP平台获取理气化痰活血方12味中药的化学成分和靶点，利用TTD、Drugbank、DisGeNET数据库筛选心脏X综合征的靶点，将心脏X综合征靶点与药物靶点进行Venn分析，筛选两者共同靶点，利用Cytoscape3.5.1软件构建化合物-靶点、化合物-基因靶点-通路-疾病网络，应用string平台构建蛋白-蛋白相互作用网络。最后，通过DAVID（6.8）在线分析数据工具对共同靶点进行GO富集分析和KEGG通路富集分析。[结果] 共筛选出理气化痰活血方165种化学成分及与治疗心脏X综合征相关的关键靶点35个，靶点主要涉及炎症反应、细胞增殖与凋亡、物质代谢、激素分泌等过程，可能通过调节TNF、MAPK、IL-17、TLRs、NLRs、HIF-1、TCR、PI3K-Akt等信号通路治疗心脏X综合征。[结论] 理气化痰活血方主要以抗炎、抗氧化、抗心肌缺血、保护血管内皮功能、调节代谢等多成分、多靶点和多途径协同作用治疗心脏X综合征，为其临床应用及进一步研究其药效作用机制奠定基础。

Abstract:

[Objective] Using network pharmacology to analyze the molecular mechanism of qi-regulating, phlegm-resolving, and blood-promoting prescription in the treatment of Cardiac Syndrome X. [Methods] We obtained the chemical constituents and targets of 12 traditional Chinese medicines of qi-regulating, phlegm-resolving, and blood-promoting prescription from the TCMSP platform, and the targets of Cardiac Syndrome X were obtained from TTD, Drugbank and DisGeNET databases. To screen the common targets, we used Venn analysis to analyze the target of Cardiac Syndrome X and drug targets. Then, we used Cytoscape 3. 5. 1 software to create component-target network and component-target gene-pathway-disease network. A protein-protein interaction network was constructed through the STRING database. Finally, we performed GO enrichme nt analysis and KEGG pathway enrichment analysis on common targets by using the DAVID(6. 8) online analytical data tool. [Results] We screened out 165 chemical components of qi-regulating, phlegm-resolving, and blood-promoting prescription and 35 key targets which are related to the treatment of Cardiac Syndrome X. These key targets mainly involve processes such as inflammatory response, cell proliferation and apoptosis, substance metabolism, hormone secretion, regulating signaling pathways such as TNF, MAPK, IL-17, TLRs, NLRs, HIF-1, TCR, and PI3K-Akt to treat the Cardiac Syndrome X. [Conclusion] The results showed that qi-regulating, phlegm-resolving, and blood-promoting prescription mainly treats cardiac syndrome X with multi-component, multi-target and multi-channel synergistic effects such as anti-inflammatory action, anti-oxidation, anti-ischemia, protection of vessel endothelium, regulation of metabolise. This research research will lay the foundation for clinical application and further study of its pharmacodyna-micmechanism.