| 摘要: |
| [目的] 建立同时测定经典名方济川煎中松果菊苷、毛蕊花糖苷、藁本内酯、柚皮苷、新橙皮苷及异阿魏酸的HPLC方法，为济川煎质量控制提供科学依据。[方法] 采用Waters WAT054275 C18色谱柱（4.6 mm×250 mm， 5 μm）；流动相为乙腈-0.1%磷酸水溶液，梯度洗脱；流速1.0 mL/min；柱温：30 ℃；检测波长310 nm；进样量为10 μL。[结果] 松果菊苷、毛蕊花糖苷、藁本内酯、柚皮苷、新橙皮苷及异阿魏酸在5.18～51.84 μg/mL（r=0.999 6）、3.60～36.00 μg/mL（r=0.999 4）、11.22～112.20 μg/mL（r=0.999 6）、20.80～208.00 μg/mL（r=0.999 1）、14.80～148.00 μg/mL（r=0.999 9）、0.98～9.80 μg/mL（r=0.999 3）浓度范围内线性关系良好，6种测定成分的平均加样回收率分别为100%、99.2%、99.0%、99.5%、99.4%、98.1%，RSD分别为0.95%、0.36%、0.56%、0.92%、1.11%、3.42%。[结论] 该方法稳定、专属性强、灵敏度高、重复性好，为经典名方济川煎的质量评价提供参考。 |
| 关键词: 经典名方 济川煎 多成分 含量测定 HPLC |
| DOI：10.11656/j.issn.1673-9043.2022.02.22 |
| 分类号:R284.2 |
| 基金项目:国家自然科学基金资助项目（81973557）；天津市自然科学基金重点项目（20JCZDJC00010）；国家科技重大专项（2018ZX09737-019）；国家科技重大专项（2018ZX09303-024）。 |
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| Simultaneous determination of multicomponent in classical herbal prescription Jichuan Decoction by HPLC method |
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CAI Nan1,2, CAO Ningning1, ZHANG Wanjing1, PEI Shuai1, LI Xiaoxuan1, XIAO Xuefeng1, LIU Changxiao3
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1.School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.Traditional Chinese Medicine Research Center, Tasly Holding Group Co., Ltd., Tianjin 300410, China;3.State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China
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| Abstract: |
| [Objective] To establish an HPLC method for simultaneous determination of echinacoside,verbascoside,ligustilide,naringin,neohesperidin and isoferulic acid in Jichuan Decoction,and provide a scientific basis for its quality control. [Methods] The HPLC separation was performed on Waters WAT054275 C18 chromatographic column (4.6 mm×250 mm,5 μm),with gradient elution using the mobile phase consisting of 0.1% phosphoric acid solution and acetonitrile at the flow rate of 1.0 mL/min. The column temperature was 30℃,detection wave-length was 310 nm and the injection volume was 10 μL. [Results] The calibration curve was liner within the range of 5.18~51.84 μg/mL (r=0.999 6) for echinacoside,3.60~36.00 μg/mL(r=0.999 4) for verbascoside,11.22~112.20 μg/mL(r=0.999 6) for ligustilide,20.80~208.00 μg/mL(r=0.999 1) for naringin,14.80~148.00 μg/mL (r=0.999 9) for neohesperidin,0.98~9.80 μg/mL(r=0.999 3) for isoferulic acid,and the average recovery were 100%,99.2%,99.0%,99.5%,99.4%, and 98.1%;with RSD were 0.95%,0.36%,0.56%,0.92%,1.11%,and 3.42%. [Conclusion] The method is stable,highly specific,accurate,reproducible,and can provide reference for the quality evaluation of classical famous prescription Jichuan Decoction. |
| Key words: classical famous prescription Jichuanjan Decoction multicomponent determination of contents HPLC |
