| 摘要: |
| [目的] 对19批不同基源淫羊藿和4批巫山淫羊藿中的主要有效成分进行含量测定比较。[方法] 采用LC30超高效液相色谱仪，Shim-pack GISS C18色谱柱（100 mm×2.1 mm，1.9 μm）梯度洗脱，对淫羊藿和巫山淫羊藿中新绿原酸、绿原酸、隐绿原酸、木兰花碱、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、箭藿苷A、箭藿苷B、宝藿苷I共11种成分进行含量检测，分析不同淫羊藿药材中各成分含量范围，并用主成分分析法（PCA）对不同淫羊藿进行区分。[结果] 淫羊藿、朝鲜淫羊藿、柔毛淫羊藿、巫山淫羊藿中淫羊藿苷含量范围分别为2.04~12.49 mg/g、2.39~11.32 mg/g、6.54~9.32 mg/g、5.16~17.28 mg/g，朝藿定A为0.43~2.11 mg/g、0.17~3.31 mg/g、1.85~2.49 mg/g、0.16~3.16 mg/g，朝藿定B为0.22~13.86 mg/g、1.84~4.22 mg/g、2.48~3.35 mg/g、1.77~4.61 mg/g，朝藿定C为0~8.21 mg/g、0~2.62 mg/g、15.50~21.15 mg/g、16.79~34.96 mg/g，绿原酸含量范围分别为0.17~1.19 mg/g，0.87~1.95 mg/g，0.35~0.77 mg/g，0~1.42 mg/g。主成分分析显示，朝鲜淫羊藿、淫羊藿、柔毛淫羊藿三者有较大差别。[结论] 建议单独建立柔毛淫羊藿的质量控制检测指标。 |
| 关键词: 淫羊藿 超高效液相色谱 含量测定 主成分分析 |
| DOI：10.11656/j.issn.1673-9043.2022.02.23 |
| 分类号:R284.2 |
| 基金项目:天津市自然科学基金项目（18JCYBJC28700）。 |
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| Determination of 11 effective components in Epimedium from different primordium by UPLC |
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CHENG Liyuan1, HUO Xiangxiang1, YANG Li1, ZHANG Yue1,2, ZHOU Kun1,2
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1.Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China
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| Abstract: |
| [Objective] Determined and compared the content of 11 main effective components in 19 batches of Epimedium in different primordium and 4 batches of Wushan Epiepiepicabin. [Methods] The content of Neochlorogenic acid,Chlorogenic acid,Cryptochlorogenic acid,Magnolia alkaloid,Epimedine A,Epimedine B,Epimedin C,Icariin,Epimedium A,Epimedin B and Baohuoside I in Epimedium and Wushan Epiepiepicabin were determined by LC30 ultra performance liquid chromatography and Shim-pack GISS C18 column(100 mm×2.1 mm,1.9 μm) gradient elution,and the principal component analysis(PCA) was used to distinguish the four basic sources of Epimedium. [Results] The content detection results showed that the content range of icariin in Epiepimedium,Korean Epimedium,Epimedium pubescens and Wushan Epiepiepicabin was 2.04~12.49 mg/g,2.39~11.32 mg/g,6.54~9.32 mg/g,and 5.16~17.28 mg/g;the Epimedine A was 0.43~2.11 mg/g,0.17~3.31 mg/g,and 1.85~2.49 mg/g,the Epimedine B was 0.22~13.86 mg/g,1.84~4.22 mg/g,2.48~3.35 mg/g and 1.77~4.61 mg/g;the Epimedin C was 0~8.21 mg/g,0~2.62 mg/g,15.50~21.15 mg/g and 16.79~34.96 mg/g,the Chlorogenic acid was 0.17~1.19 mg/g,0.87~1.95 mg/g,0.35~0.77 mg/g and 0~1.42 mg/g. PCA showed that there was obviously different among Korean Epimedium,Epimedium and Epimedium pubescens. [Conclusion] It is recommended to establish a separate quality control test index for Epimedium pubescens. |
| Key words: Epimedium UPLC determination of content PCA |
