| 摘要: |
| [目的] 夏枯草(Prunella vulgaris L.)在临床中已经应用于治疗自身免疫性甲状腺炎(AIT), 但是机制尚不明确。本研究通过建立实验性自身免疫性甲状腺炎(EAT)大鼠模型, 研究夏枯草水提液对EAT大鼠5-羟色胺(5-HT)、5-羟色胺转运体(SERT)和Th17的作用。[方法] 水提法提取夏枯草的有效成分并采用高效液相色谱法(HPLC)对其主要活性成分进行分析; 采用皮下注射甲状腺球蛋白(Tg)联合高碘水喂养的方式建立EAT大鼠模型, 随后将EAT大鼠分为模型对照组(EAT), 夏枯草低剂量组(E-LOW), 夏枯草中剂量组(E-MID), 夏枯草高剂量组(E-HIG), 连续灌胃12周。采用酶联免疫吸附检测法(ELISA)检测大鼠血清中辅助性T细胞17(Th17)和细胞因子水平; HE染色观察大鼠甲状腺形态; 流式细胞术检测大鼠外周血单个核细胞(PBMCs)中CD4+IL17A+(Th17)淋巴细胞的水平; qRT-PCR检测脾脏中SERT水平; ELISA法检测大鼠脾脏和血清中5-HT以及血清中IL-17A, IL-6含量。[结果] 迷迭香酸(Rosmarinic Acid)是夏枯草提取物的主要成分, 夏枯草水提液治疗12周可显著降低大鼠血清TgAb(P<0.05或P<0.01), 减小甲状腺体积(P<0.01), 减轻甲状腺内炎性浸润, 降低外周血Th17比例(P<0.01)和血清IL-17A, 抑制大鼠脾脏中SERT水平(P<0.01), 提高脾脏和血清5-HT水平(P<0.01)。[结论] 夏枯草可通过提高EAT大鼠中5-HT含量, 抑制Th17细胞分化, 发挥调节免疫作用, 该研究为夏枯草治疗EAT提供新的思路。 |
| 关键词: 自身免疫性甲状腺炎 5-羟色胺 5-羟色胺转运体 Th17 |
| DOI：10.11656/j.issn.1673-9043.2022.04.16 |
| 分类号:R581.4 |
| 基金项目: |
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| Prunella vulgaris L. attenuates experimental autoimmune thyroiditis by inducing5-hydroxytryptamine expression and inhibiting Th17 T cell |
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ZHANG Hong1, GENG Hui2, QU Haili3, ZHONG Xia1
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1.Department of General Practice, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China;2.Department of Pharmacy, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China;3.Nursing Department, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China
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| Abstract: |
| [Objective] Prunella vulgaris L. has been used in the treatment of autoimmune thyroiditis(AIT), but the mechanism is not clear. In this study, experimental autoimmune thyroiditis (EAT) rats model was built to study the effects of Prunella vulgaris L. aqueous extract on 5-hydroxytryptamine (5-HT), 5-HT transporter (SERT) and Th17.[Methods] The aqueous extract of Prunella vulgaris L. were extracted and analyzed by HPLC; The EATs were established by subcutaneous injection of thyroglobulin (Tg) combined with high iodine water. Then the rats were divided into model control group (EAT), low-dose Prunella vulgaris L. group (E-Low), medium dose Prunella vulgaris L. group (E-MID) and high-dose Prunella vulgaris L. group (E-HIG) for 12 weeks. The levels of serum thyroglobulin antibody (TgAb) and cytokines were detected by enzyme-linked immunosorbent assay (ELISA); thyroid morphology was observed by HE staining; The levels of CD4+ IL17A+ (Th17) lymphocytes in spleen were detected by flow cytometry; SERT level in spleen was detected by qRT-PCR; The levels of 5-HT in spleen and serum, IL-17A and IL-6 in serum were detected by ELISA.[Results] Rosmarinic acid is the main component of Prunella vulgaris L. aqueous extract. After 12 weeks of treatment, Prunella vulgaris L. significantly reduce serum TgAb (P<0.05 or P<0.01), thyroid volume (P<0.01), inflammatory infiltration in thyroid gland, Th17 ratio in Peripheral blood (P<0.01) and serum IL-17A, moreover, it effectively inhibited SERT level in spleen (P<0.01), and increased 5-HT in spleen and serum (P<0.01).[Conclusion] Prunella vulgaris L. can inhibit the differentiation of Th17 cells by increasing the 5-HT content in EAT rats to regulate immunity. This research provides a new idea for the treatment of EAT with Prunella vulgaris L. |
| Key words: autoimmune thyroiditis 5-hydroxytryptamine 5-HT transporter Th17 |
