| 摘要: |
| [目的] 红景天苷是从景天科植物大株红景天的干燥根及根茎或干燥全草中提取的一种化合物，具有多种药理作用。研究旨在探讨红景天苷下调角蛋白家族成员17（KRT17）影响紫杉醇对卵巢癌细胞的化疗敏感性。[方法] 红景天苷、紫杉醇单独或联用处理SKOV-3细胞，将细胞随机分为4组：对照组、红景天苷单独处理组、紫杉醇单独处理组和联合处理组，Cell Counting Kit-8（CCK-8）法检测细胞毒性，5-乙炔基-2’-脱氧尿苷（EDU）检测细胞增殖，Hoechst 33258检测细胞凋亡。免疫荧光法检测自噬体形成情况，蛋白免疫印迹法检测KRT17和细胞自噬相关蛋白表达水平。[结果] 与对照组比较，红景天苷、紫杉醇单独处理组随浓度的升高，对SKOV-3细胞的毒性增强，细胞增殖数、P62蛋白表达降低，细胞凋亡率、LC3荧光斑点数、LC3-Ⅱ/LC3-I比值、Beclin1蛋白表达升高。且红景天苷与紫杉醇协同处理效果优于红景天苷、紫杉醇单独处理组，可能是红景天苷通过下调KRT17蛋白表达实现的。[结论] 红景天苷下调KRT17增加紫杉醇对卵巢癌细胞的化疗敏感性。 |
| 关键词: 卵巢癌 红景天苷 紫衫醇 角蛋白家族成员17 |
| DOI：10.11656/j.issn.1673-9043.2022.05.16 |
| 分类号:R737.31 |
| 基金项目:湖北省自然科学基金项目（2017CFB325）。 |
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| Salidroside down-regulates KRT17 and increases the sensitivity of paclitaxel to chemotherapy in ovarian cancer cells |
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LIU Hua1, ZHOU Lingling1, WEI Dandan1, XU Yiyan1, ZHANG Hui2
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1.Obstetrics and Gynecology Department of Yicheng People's Hospital, Yicheng 441400, China;2.Xiangyang Central Hospital, Obstetrics and Gynecology Department of Affiliated Hospital of Hubei University of Arts and Sciences, Xiangyang 441021, China
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| Abstract: |
| [Objective] Salidroside is a compound extracted from dried roots and rhizomes or dried whole grasses of large plants of the family rhodiola rosea. It has a variety of pharmacological effects. The aim of this study was to investigate the effect of salidroside's down-regulation of Keratin17(KRT17) on the chemotherapy sensitivity of paclitaxel to ovarian cancer cells.[Methods] SKOV-3 cells were treated with salidroside and paclitaxel alone or in combination. The cells were randomly divided into four groups:control group, salidroside alone, paclitaxel alone and in combination. Cell Counting Kit 8(CCK-8) was used to detect cytotoxicity, 5-acetyl-2'-deoxyuridine(EDU) was used to detect cell proliferation, and Hoechst 33258 was used to detect apoptosis. The formation of autophage was detected by immunofluorescence method, and the expression of KRT17 and autophagy related proteins were detected by Western Blot.[Results] Compared with DMSO group, individual treatment group increased with the concentration of salidroside, paclitaxel enhanced on SKOV-3 cell toxicity, number of cell proliferation, P62 protein expression decreased, cell apoptosis rate, the number of LC3 fluorescence spots, LC3-Ⅱ/LC3-I ratio, and Beclin1 expression were increased. Moreover, the synergistic effect of salidroside and paclitaxel was better than salidroside and paclitaxel alone, which may be achieved by salidroside down-regulating the expression of KRT17 protein.[Conclusion] Salidroside down-regulates KRT17 and increases the chemotherapy sensitivity of paclitaxel to ovarian cancer cells. |
| Key words: ovarian cancer salidroside paclitaxel Keratin17 |
