| 摘要: |
| [目的] 利用快速、灵敏的UHPLC-Q-TOF-MS/MS分析技术对吴茱萸药材的化学成分定性分析。[方法] 色谱条件为ZORBAX Eclipse plus C18色谱柱（2.1 mm×100 mm，1.8 μm），水（0.2%甲酸）-乙腈（0.2%甲酸）为流动相，流速为0.3 mL/min，柱温30℃；采用电喷雾离子源，正负离子扫描检测，经PeakView软件对数据进行分析。[结果] 共鉴定出64个化合物，包括生42个生物碱、8个柠檬苦素、8个黄酮苷、3个苯丙素和3个其他类型化合物。[结论] 本实验方案能快速、精准地对吴茱萸的化学成分进行分析，为研究吴茱萸毒性成分和药效成分打下基础。 |
| 关键词: 吴茱萸 UHPLC-Q-TOF-MS/MS 成分分析 |
| DOI：10.11656/j.issn.1673-9043.2023.02.15 |
| 分类号:R284.1 |
| 基金项目:国家自然科学基金项目（81560648）；河北省中医药管理局课题项目（2018336）。 |
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| Analysis of chemical constituents from Evodia rutaecarpa by UHPLC-Q-TOF-MS/MS |
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LI Ming1, YUAN Jinbin2, ZHANG Xun3, FENG Hao3, REN Xiaojing3
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1.Tangshan People's Hospital, Tangshan 063000, China;2.Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;3.Tangshan Union Hospital, Tangshan 063000, China
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| Abstract: |
| [Objective] A rapid and sensitive UHPLC-Q-TOF-MS/MS method was used for qualitative analysis of chemical constituents of Evodia rutaecarpa.[Methods] The chromatographic conditions were ZORBAX Eclipse plus C18 column (2.1 mm×100 mm, 1.8 μm) with water (0.2% formic acid)-acetonitrile (0.2% formic acid) as mobile phase at the flow rate of 0.3 mL/min and column temperature of 30℃. Using electrospray ion source, positive and negative ion scanning detection, data in this study analysis by PeakView software.[Results] A total of 64 compounds were identified, including 42 alkaloids, 8 limonin, 8 flavone glycosides, 3 phenylpropanoids and 3 other compounds.[Conclusion] This experimental method can quickly and accurately analyze the chemical constituents of Evodia rutaecarpa, and lay a foundation for the study of the toxic components and pharmacodynamic components of Evodia rutaecarpa. |
| Key words: Evodia rutaecarpa UHPLC-Q-TOF-MS/MS component analysis |
