基于HS-GC-IMS和HS-SPME-GC-MS技术对清感秋饮中挥发性成分的全面表征

Comprehensive characterization of volatile organic compounds in Qinggan Qiuyin based on HS-GC-IMS and HS-SPME-GC-MS

关键词:  清感秋饮  顶空-气相色谱-离子迁移谱  顶空-固相微萃取-气质联用技术  挥发性成分

Key words:  Qinggan Qiuyin  headspace gas chromatography-ion mobility spectrometry  headspace solid phase microextraction gas chromatography-mass spectrometry  volatile organic compounds

摘要:

[目的] 对清感秋饮和菊花中的挥发性成分进行全面表征。[方法] 基于顶空-气相色谱-离子迁移谱（HS-GC-IMS）技术构建清感秋饮和菊花的挥发性成分指纹图谱，确定共有峰；采用顶空-固相微萃取-气质联用（HS-SPME-GC-MS）技术对清感秋饮中挥发性成分进行定性定量分析。[结果] HS-GC-IMS方法鉴定出41种挥发性成分，HS-SPME-GC-MS方法鉴定出85种挥发性成分，其中6种挥发性成分在两种方法中均被检测出。此外，HS-SPME-GC-MS定量方法表明：酮类成分、烃类成分、醇类成分、酯类成分和环类成分分别占总挥发性成分的51.97％、18.15％、13.05％、6.17％和4.41％，以上几类成分占到总挥发性成分的90％以上。[结论] 定性结合定量分析从清感秋饮中共鉴定出120种挥发性成分，其中，紫苏属酮、异白苏烯酮、（Z）-乙酸菊酯、β-石竹烯等可能为其主要药效成分。

Abstract:

[Objective] The volatile organic compounds(VOCs) in Qinggan Qiuyin and chrysanthemum were comprehensively analyzed.[Methods] Headspace gas chromatography-ion mobility spectrometry(HS-GC-IMS) technology was used to construct the fingerprint of VOCs of Qinggan Qiuyin and chrysanthemum, and the common peaks were identified;Headspace solid phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) technology was used for qualitative and quantitative analysis of VOCs in Qinggan Qiuyin.[Results] The 41 VOCs were identified by HS-GC-IMS, and 85 VOCs were identified by HS-SPME-GC-MS, among which 6 overlapping chemical components were detected by both methods. The quantitative results of HS-SPME-GC-MS showed that ketones, hydrocarbons, alcohols, esters and rings accounted for 51.97%, 18.15%, 13.05%, 6.17% and 4.41% of the total VOCs, and the above VOCs accounted for more than 85%.[Conclusion] A total of 120 VOCs were identified from Qinggan Qiuyin by qualitative and quantitative analysis, among which perilla ketone, isoegomaketone, (Z)-chrysanthenyl acetate, β-caryophyllene and so on may be its main pharmacodynamic components.