| 摘要: |
| [目的] 分析经淫羊藿苷(ICA)刺激后,人根尖牙乳头干细胞(hSCAPs)增殖及骨向分化的作用机制。[方法] hSCAPs体外培养至第3代后与不同浓度的ICA进行温育。2 d后,采用qRT-PCR和Western Blotting技术在mRNA和蛋白水平上分别检测hSCAPs的骨向分化相关因子骨保护素(OPG)和核因子-κB受体激活因子配体(RANKL)的表达;并通过CCK-8检测hSCAPs受不同浓度的ICA诱导不同时间的增殖情况。[结果] 根尖乳头分离出的hSCAPs在体外培养后,流式染色技术检测表面蛋白CD31、CD90和CD146细胞表达率分别为4.03%、96.18%和95.42%。与对照组相比,ICA的体外刺激显著促进了hSCAPs中OPG的表达(P<0.05),降低了RANKL的mRNA转录和翻译(P<0.05),促进了hSCAPs的增殖分化(P<0.05)。当浓度为0.1 mol/L时,ICA对hSCAPs的调控作用最强。[结论] ICA对hSCAPs细胞增殖和骨向分化的促进作用为临床上使用hSCAPs进行牙病治疗提供理论指导。 |
| 关键词: 淫羊藿苷 人根尖牙乳头干细胞 骨向分化 细胞增殖 |
| DOI：10.11656/j.issn.1673-9043.2023.04.14 |
| 分类号:R285.5 |
| 基金项目:深圳市龙华区医疗卫生机构区级科研项目(2020110) |
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| Investigate the effect of icariin on proliferation and osteogenic differentiation of human apical papillary stem cells |
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XIE Erting1, HUANG Bailan2, LI Wenlong3, LI Xiaoyu3
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1.Department of Orthodontics, Shenzhen Longhua District People's Hospital, Shenzhen 518109, China;2.Department of Periodontology, Shenzhen Longhua District People's Hospital, Shenzhen 518109, China;3.Department of Oral and Maxillofacial Surgery, Shenzhen Longhua District People's Hospital, Shenzhen 518109, China
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| Abstract: |
| [Objective] To analyze the mechanism of human apical dental papilla stem cells(hscaps) proliferation and osteo differentiation after stimulation by Icariin(ICA).[Methods] The hSCAPs were incubated with different concentrations of ICA after cultured in vitro to the third passage for 2 d,and the osteo differentiation related factor osteoprotegerin of hSCAPs was detected at the mRNA and protein levels by QRT PCR and Western blotting,respectively (OPG) and nuclear factor-κ B receptor activator ligand (RANKL) expression;and the proliferation of hSCAPs incubated with different concentrations of ICA for different times was detected by CCK-8.[Results] After the isolation of hSCAPs from apical papilla in vitro,the expression of stem cell surface markers CD31,CD90,and CD146 cell expression rate was 4.03%,96.18%,and 95.42%,respectively,as examined by flow staining. Compared with the control group,In vitro stimulation with ICA significantly promoted the expression of OPG(P<0.05),decreased the mRNA transcription and translation of RANKL(P<0.05),and promoted the proliferation and differentiation of hSCAPs(P<0.05). The effect of ICA on the regulation of hSCAPs was the strongest when the concentration was 0.1 mol/L.[Conclusion] The promoting effects of ICA on cell proliferation and bone to differentiation of hSCAPs provide theoretical guidance for the clinical use of hSCAPs for dental disease treatment. |
| Key words: ICA hSCAPs osteolineage differentiation cell proliferation |
