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| 扩增子测序技术在中药分子鉴定中的应用 |
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张悦1,2, 张婷婷1,2, 邢志美1,2, 徐流巍1,2, 田晓轩1,2
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1.天津中医药大学, 组分中药国家重点实验室, 天津 301617;2.现代中医药海河实验室, 天津 301617
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| 摘要: |
| 中药鉴定是保证中药用药安全的关键环节。中药分子鉴定通过分析遗传物质的差异实现鉴定，弥补了传统鉴定方法主观性较强的不足，成为中药鉴定方法的有力补充。扩增子测序技术通过对特定片段进行扩增，结合不同测序技术实现物种鉴定。文章对扩增子测序技术在生物鉴定中的优势、扩增子测序技术在中成药及中药材中的应用，以及扩增子测序技术在中药鉴定中的关键技术步骤进行总结梳理，分析扩增子测序技术在中药分子鉴定中的应用现状及未来发展，为后续中药分子鉴定工作提供研究思路。 |
| 关键词: 扩增子测序 中药鉴定 中药分子鉴定 |
| DOI：10.11656/j.issn.1673-9043.2024.10.11 |
| 分类号:R282.5 |
| 基金项目:天津中医药大学研究生科研创新项目（YJSKC-20221036）;天津市研究生科研创新项目（2022SKY227）;天津中医药大学第十三届大学生科技创新基金项目（KJ15）。 |
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| The application of amplicon sequencing technology in molecular identification of traditional Chinese medicine |
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ZHANG Yue1,2, ZHANG Tingting1,2, XING Zhimei1,2, XU Liuwei1,2, TIAN Xiaoxuan1,2
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1.State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China
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| Abstract: |
| Traditional Chinese mediciｎｅ （TCM） identification is a crucial step in ensuring the safety of TCM use. Molecular identification of TCM involves analyzing genetic material differences， which helps to overcome the subjectivity of traditional identification methods and serves as a complement to TCM identification. The amplicon sequencing technique amplifies specific gene fragments and combines various sequencing technologies for species identification. This review summarizes and analyzes the advantages of amplicon sequencing technology in biological identification， its applications in TCM and Chinese patent medicine， and the key technical steps in TCM identification using amplicon sequencing. It also examines the current status and future development of amplicon sequencing technology in molecular identification of TCM， providing research insights for future work in this field. |
| Key words: amplicon sequencing traditional Chinese medicine identification molecular identification of traditional Chinese medicine |
