| 摘要: |
| [目的] 评价蓟黄素通过调控STING/NF-κB信号通路对小鼠胶原诱导性关节炎（CIA）的影响。[方法] 取40只SPF级雌性C57BL/6小鼠平均分为空白对照组（Sham）、模型组（CIA）、甲氨蝶呤组（MTX，1 mg/kg）和蓟黄素组（JHS，100 mg/kg），通过胶原免疫法建立小鼠类风湿性关节炎模型。药物干预4周后实验结束，分别检测后爪肿胀度、血清中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、白细胞介素6（IL-6）、白细胞介素10（IL-10）、丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性，观察左侧膝关节形态学变化，测定右侧膝关节STING/NF-κB通路关键蛋白表达水平。[结果] 与CIA组相比，MTX组和JHS组小鼠后爪肿胀度明显降低（P<0.01），血清TNF-α、IL-1β、IL-6、IL-10和MDA水平明显降低，而SOD活性明显增加（P<0.01），膝关节炎症细胞浸润明显减少，软骨组织损伤明显减轻，膝关节STING和p-NF-κB p65/NF-κB蛋白表达水平明显降低（P<0.01）。[结论] 蓟黄素可明显改善类风湿性关节炎，其机制可能通过调控STING/NF-κB信号通路减轻炎症和氧化应激损伤有关。 |
| 关键词: 蓟黄素 小鼠 类风湿性关节炎 甲氨蝶呤 STING/NF-κB信号通路 |
| DOI：10.11656/j.issn.1673-9043.2024.11.05 |
| 分类号:R285.5 |
| 基金项目:广东省中医药局中医药科研项目(20222105);湛江市科技发展专项资金竞争性分配项目(2021A05063)。 |
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| The effect of cirsimaritin on rheumatoid arthritis in mice based on STING/NF-κB signaling pathway |
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HUANG Chengshuo1, LIN Xian2, CHEN Ju3, ZHENG Jinchang1, LIANG Zhen1, GUO Weixiong1, CHEN Jiming1
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1.Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China;2.Department of Ultrasound Medicine Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China;3.Department of Pharmacy, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China
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| Abstract: |
| [Objective] To evaluate the effect of thistle flavescens on collagen-induced arthritis（CIA） in mice by regulating STING/NF-κB signaling pathway. [Methods] Forty SPF grade female C57BL/6 mice were divided into blank control group（Sham）, model group（CIA）, methotrexate group（MTX, 1 mg/kg） and cirsimaritin group（JHS, 100 mg/kg）. The mice rheumatoid arthritis models were established by collagen immunoassay. After 4 weeks of drug intervention, the experiment was completed. Swelling degree of the hind PAWS, levels of TNF-α, IL-1β, IL-6, IL-10, MDA and SOD activity in serum were detected, morphological changes of the left knee joint were observed, and the expression level of STING/NF-κB pathway key proteins in the right knee joint was measured. [Results] Compared with CIA group, the swelling degree of hind PAWS in MTX group and JHS group was significantly decreased（P<0.01）, the levels of TNF-α, IL-1β, IL-6, IL-10 and MDA in serum were significantly decreased, while the activity of SOD was significantly increased（P<0.01）, the inflammatory cell infiltration of knee joint was significantly decreased, and the cartilage tissue damage was significantly alleviated. The expression levels of STING and P-NF-κB p65/NF-κB protein in knee joint were significantly decreased（P<0.01）. [Conclusion] Cirsimaritin can significantly improve rheumatoid arthritis, and its mechanism may be related to reducing inflammation and oxidative stress damage by regulating STING/NF-κB signaling pathway. |
| Key words: cirsimaritin mouse methotrexate STING/NF-κB signaling pathway |
