虎杖苷与马鞭草苷改善环磷酰胺诱导小鼠免疫抑制的作用比较

Comparison of polydatin and verbenalin in ameliorating cyclophosphamide-induced immunosuppression in mice

关键词:  虎杖苷  马鞭草苷  环磷酰胺  免疫抑制  免疫功能

Key words:  polydatin  verbenalin  cyclophosphamide  immunosuppressed  immunological function

摘要:

[目的] 比较虎杖苷和马鞭草苷对环磷酰胺（CY）诱导的免疫抑制小鼠的免疫功能的改善作用。[方法] 选取雄性BALB/C小鼠24只，随机分为4组：空白组、模型组、虎杖苷组和马鞭草苷组，每组6只。空白组采用生理盐水腹腔注射，其余各组采用CY腹腔注射构建免疫抑制模型。空白组和模型组给予纯水灌胃，虎杖苷和马鞭草苷组分别给予虎杖苷和马鞭草苷灌胃治疗；在第11天，眼球取血，检测血清中免疫球蛋白G（IgG）和免疫球蛋白M（IgM）的水平。分离胸腺和脾脏称重计算胸腺和脾脏指数；HE染色考察小鼠肝、脾和胸腺的病理变化。RT-qPCR法检测小鼠脾脏中白细胞介素-2（IL-2）和白细胞介素-4（IL-4）水平。用流式细胞术统计脾脏CD4⁺和CD8⁺ T细胞数量。用CCK-8法考察脾B淋巴细胞增殖能力。[结果] 虎杖苷和马鞭草苷作用一致的是，均可显著上调免疫抑制小鼠脾脏指数（P<0.01）和脾组织中IL-2水平（P<0.01）；均可以改善CY诱导的肝、脾和胸腺的病理损伤；显著增强脂多糖（LPS）诱导脾B淋巴细胞的增殖能力（P<0.01）。同时，虎杖苷还可显著抑制免疫抑制小鼠体质量的下降（P<0.01）；显著上调胸腺指数（P<0.01）；上调免疫抑制小鼠血清IgM（P<0.01）和IgG（P<0.01）水平；上调免疫抑制小鼠脾脏中CD4⁺和CD8⁺ T细胞数量。[结论] 虎杖苷和马鞭草苷均具有增强免疫功能作用，但虎杖苷的免疫调节作用优于马鞭草苷。

Abstract:

[Objective] Comparison of the ameliorative effects of polydatin and verbenalin on immune function in cyclophosphamide(CY)-induced immunosuppressed mice.[Methods] Twenty-four male BALB/C mice were randomly divided into four groups:control group,model group,polydatin group and verbenalin group,with six mice in each group. The control group and model group were given pure water gavage,while the polydatin and verbenalin groups were given polydatin and verbenalin gavage treatment,respectively. After 11 days,the blood was collected,and serum immunoglobulin G(IgG) and immunoglobulin M(IgM) were measured by the enzyme-linked immunosorbent assay(ELISA). The thymus and spleen were isolated and weighed,then the thymus and spleen indices were calculated. The pathological structures of the liver,spleen,and thymus were analyzed by HE staining. RT-PCR was used to detect interleukin-2(IL-2) and interleukin-4(IL-4) expression in spleen. CD4⁺ and CD8⁺ T cell counts were assessed by flow cytometry. The proliferation of splenic B cells was evaluated using the CCK-8 assay.[Results] Consistently,after polydatin or verbenalin administration,the index of the spleen and the level of IL-2 both were significantly upregulated. The pathological alteration was also much improved. The lipopolysaccharide(LPS)-induced proliferation of B cells was much enhanced. In addition,after polydatin administration,the body weight and thymus index increased significantly. The levels of IgM and IgG and the counts of CD4⁺ and CD8⁺ T cells were also upregulated.[Conclusion] Polydatin and verbenalin have certain immunomodulatory effects,and the immunomodulatory effect of polydatin is better than that of verbenalin.