腹部推拿对单纯性肥胖模型大鼠摄食量及结肠PYY与GLP-1表达影响

Effects of abdominal acupressure on food intake and colonic PYY and GLP-1 expression in rats with simple obesity model

关键词:  腹部推拿  单纯性肥胖  PYY  GLP-1  摄食量  体质量

Key words:  abdominal acupressure  simple obesity  PYY  GLP-1  food intake  body weight

摘要:

[目的] 探讨腹部推拿对单纯性肥胖大鼠模型结肠肽YY（PYY）与胰高血糖素样肽-1（GLP-1）表达影响，分析腹部推拿调控单纯性肥胖的可能作用机制。[方法] 24只SD雄性大鼠，随机数字表法随机分为3组：空白组、模型组、推拿组，各8只。模型组、推拿组予以自制高脂饲料，建立单纯性肥胖动物模型。推拿组予以腹部推拿法（摩中脘穴、推任脉），20 min/次，1次/d，干预14 d。模型组仅施予推拿组同等时间束缚。空白组不予造模及干预。干预结束后，检测3组大鼠体质量、摄食量、体长、Lee’s指数等评价其生理指标变化情况。采用酶联免疫吸附测定法（ELISA）检查大鼠肠道中PYY与GLP-1的表达。应用HE染色法观察肠道结肠组织形态结构变化情况。[结果] 与自身前后相比，推拿组大鼠体质量、摄食量下降（P＜0.01）；与模型组相比，推拿组脂肪系数降低（P＜0.01），PYY、GLP-1表达升高（P＜0.01）；HE检测显示，模型组大鼠结肠腺体结构排列混乱，隐窝萎缩变形，杯状细胞数量少，有慢性炎症细胞浸润。经腹部推拿后，结肠组织形态趋于恢复正常状态。[结论] 腹部推拿可能通过改善单纯性肥胖大鼠肠道PYY、GLP-1表达，调节胃肠功能，抑制食欲，制造摄入-消耗的负能量平衡状态，达到减脂减重的作用。

Abstract:

[Objective] To explore the effect of abdominal nudging on the expression of colonic PYY and GLP-1 in the model of simple obese rats,and to analyze its possible mechanism of action.[Methods] 24 SD male rats were randomly divided into 3 groups:blank group,model group and nudging group,8 rats each. The model group and the nudging group were given homemade high-fat feed to establish simple obesity animal model. In the nudging group,abdominal nudging was applied to the abdomen[rubbing Zhongwan(RN12) and pushing Renmai(CV)] for 20 min/times,1 day for 14 d. The model group was only given the same time restraint as that of the nudging group. The blank group was not modeled or intervened. At the end of the intervention,the body mass,food intake,body length and Lee's index of the rats in the three groups were examined to evaluate the changes in their physiological indexes. Enzyme-linked immunosorbent assay(ELISA) was used to examine the levels of Peptide YY(PYY) and Glucagon Like Peptide-1(GLP-1) in the intestines of rats. HE staining was applied to observe the morphological and structural changes of intestinal colon tissue.[Results] Compared with the rats before and after their own,the body mass and food intake of the nudging group decreased(P<0.01);compared with the model group,the fat coefficient of the nudging group decreased(P<0.01),and the expression of PYY and GLP-1 was elevated(P<0.01);the HE test showed that the structure of the colonic glands of the model group was disorganized,with crypts shrinking and deforming,and the number of cup cells was small,and there was infiltration of chronic inflammatory cells. After abdominal acupressure,the morphology of colon tissue tended to return to the normal state.[Conclusion] Abdominal acupressure may improve the expression of intestinal PYY and GLP-1,regulate the gastrointestinal function,inhibit appetite,and create a negative energy balance of intake-consumptionin purely obese rats to achieve the effect of fat reduction and weight loss.