| 摘要: |
| [目的] 建立木贼超高效液相色谱（UHPLC）指纹图谱，并结合化学计量学与多成分含量测定，为木贼的质量评价提供参考。[方法] 采用ACQUITY CSH C18色谱柱，流动相：0.1%甲酸水-乙腈，波长：270 nm，进行梯度洗脱；建立20批木贼药材指纹图谱，并对其指标成分进行含量测定；对指纹图谱进行相似度评价及层次聚类分析，利用正交偏最小二乘法判别分析筛选出差异性成分。[结果] 20批木贼的指纹图谱中含14个共有峰，相似度均大于0.992。通过与对照品比对，指认出了香草酸、山柰酚-3-O-槐二糖-7-O-葡萄糖苷、绿原酸、阿魏酸、山柰酚-3-O-槐糖苷。层次聚类分析将20批木贼分为两类，正交偏最小二乘法判别分析筛选出6个差异性标志物，并指认出3种成分（山柰酚-3-O-槐二糖-7-O-葡萄糖苷、阿魏酸、香草酸）。[结论] 建立的分析方法稳定、可靠，为中药材木贼的质量评价提供参考。 |
| 关键词: 木贼 超高效液相色谱 指纹图谱 含量测定 |
| DOI：10.11656/j.issn.1673-9043.2025.05.04 |
| 分类号:R285.5 |
| 基金项目:国家自然科学基金（82274091）。 |
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| Study on quality evaluation of Equiseti Hiemalis Herba by UHPLC fingerprint combined with multi-content determination |
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WU Tiantian1,2, ZHAO Peng1, LYU Zhenguo1, OUYANG Huizi1, HE Jun1
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1.State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China
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| Abstract: |
| [Objective] To establish ultra-high-performance liquid chromatography（UHPLC） fingerprint of Equiseti Hiemalis Herba and to comprehensively evaluate the quality of Equiseti Hiemalis Herba by combining chemometrics and multi-component content determination. [Methods] An ACQUITY CSH C18 chromatographic column was employed in this study，gradient elution was performed using 0.1% formic acid in water-acetonitrile as the mobile phase，and the detection wavelength was 270 nm. UHPLC fingerprints of 20 batches of Equiseti Hiemalis Herba were established，and the contents of components were determined. The fingerprints were evaluated by similarity analysis and hierarchical cluster analysis，and the differential components were found by orthogonal partial least squares discriminant analysis. [Results] There were 14 common peaks in the fingerprints of 20 batches of Equiseti Hiemalis Herba，and the similarity was greater than 0.992. Vanillic acid，kaempferol-3-O-sophoroside-7-O-glucoside，chlorogenic acid，ferulic acid，and kaempferol-3-O-sophoroside were identified by comparison with the standards. Hierarchical cluster analysis classified the 20 batches of Equiseti Hiemalis Herba into two categories，and orthogonal partial least squares discriminant analysis screened out six differential markers，and three components（kaempferol-3-O-sophoroside-7-O-glucoside，ferulic acid，vanillic acid） were identified. [Conclusion] The established analytical method is stable and reliable，and it can provide a reference for the quality evaluation of the Equiseti Hiemalis Herba. |
| Key words: Equiseti Hiemalis Herba UHPLC fingerprint content determination |
