小青龙汤和玉屏风散“同病异治”过敏性鼻炎的网络药理学作用机制研究

Network Pharmacology-based investigation into the mechanism of “different treatment of the same disease” in treating allergic rhinitis with Xiaoqinglong Tang and Yupinfen San

关键词:  小青龙汤  玉屏风散  过敏性鼻炎  同病异治  网络药理学

Key words:  Xiaoqinglong Tang  Yupinfen San  allergic rhinitis  different treatment of the same disease  network pharmacology

摘要:

[目的] 利用网络药理学的方法，分别研究小青龙汤和玉屏风散的活性成分、作用靶点及生物学意义，探讨其“同病异治”过敏性鼻炎的作用机制。[方法] 通过中药系统药理学数据库与分析平台（TCMSP）等中药化合物数据库分别挖掘获取小青龙汤和玉屏风散的化学成分和作用靶点；结合GeneCards、OMIM等数据库获取临床治疗过敏性鼻炎的有效作用靶点。采用Cytoscape软件分别绘制小青龙汤与玉屏风散“小青龙汤-活性成分-作用靶点-过敏性鼻炎”网络图及“玉屏风散-活性成分-作用靶点-过敏性鼻炎”网络图。通过借助STRING数据库，以及Cytoscape软件分析获得核心作用靶点。后使用Autodock-vina软件对小青龙汤与玉屏风散的主要活性成分与作用靶点进行分子对接。[结果] 网络分析结果表明，小青龙汤与玉屏风散两方共有有效成分包括槲皮素（quercetin）、山柰酚（kaempferol）等，小青龙汤特有成分有β-谷甾醇（beta-sitosterol）、柚皮素（naringenin）、豆甾醇（stigmasterol）等，玉屏风散独有成分汉黄芩素（wogonin）、7-O-甲基异微凸剑叶莎醇（7-O-methylisomucronulatol）、刺芒柄花素（formononetin）等。两方作用于过敏性鼻炎的共同核心靶点包括白介素（IL）-13、IL-4R、IL-4、IL-5、核糖核酸酶A3（RNASE3）等。[结论] 小青龙汤与玉屏风散在组方配伍当中存在明显的差异，药味数量和种类都完全不同；从分子角度，两方药物活性成分存在相同的化学成分以及两方各自独有的化学成分，因此，在临床治疗过敏性鼻炎方面共通之处与不同之处。故本研究通过对两方治疗过敏性鼻炎的相关靶点和通路进行分析，初步阐明两方“同病异治”过敏性鼻炎的科学内涵。

Abstract:

[Objective] This study employs network pharmacology to elucidate the active constituents，potential targets，and biological significance underlying the “different treatment of the same disease” approach in the management of allergic rhinitis using Xiaoqinglong Tang and Yupinfen San. [Methods] Active ingredients of Xiaoqinglong Tang and Yupinfen San were retrieved from TCMSP. Potential targets related to allergic rhinitis were obtained from databases such as GeneCards and OMIM. Cytoscape software was utilized to construct network plots for “Xiaoqinglong Tang-active ingredients-potential targets-allergic rhinitis” and “Yupinfen San-active ingredients-potential targets-allergic rhinitis.” Venn diagrams illustrating key targets of the disease and drugs were generated using the STRING database and Cytoscape. Molecular docking analyses were performed using Autodock-Vina. [Results] Screening revealed that quercetin and kaempferol are common active ingredients in both Xiaoqinglong Tang and Yupinfen San. Xiaoqinglong Tang includes exclusive ingredients such as beta-sitosterol，naringenin，stigmasterol，while Yupinfen San contains unique ingredients like wogonin，7-O-methylisomucronulatol，formononetin. Potential targets like IL13，IL4R，IL4，IL5，RNASE3 are shared between Xiaoqinglong Tang and Yupinfen San. [Conclusion] Xiaoqinglong Tang and Yupinfen San exhibit noticeable differences in herb dosage and quantity. From a molecular perspective，both prescriptions share common active ingredients and feature distinct ones. This suggests that while there are similarities，there are also differences in the treatment of allergic rhinitis. This study employs network pharmacology to explore the active ingredients，potential targets，and biological significance of Xiaoqinglong Tang and Yupinfen San，providing insight into the “different treatment of the same disease” paradigm in allergic rhinitis management.