基于指纹图谱和多成分含量测定的莲房质量评价研究

Study on quality evaluation of Nelumbinis Receptaculum based on fingerprint and multi-component quantification

关键词:  莲房  指纹图谱  含量测定  化学计量学  质量评价

Key words:  Nelumbinis Receptaculum  fingerprint  content determination  chemometrics  quality evaluation

摘要:

[目的] 建立莲房Nelumbinis Receptaculum的高效液相色谱指纹图谱及多成分含量测定方法，结合化学计量学分析评价不同产地莲房药材质量。[方法] 采用Agilent ZORBAX SB-C18色谱柱（250 mm×4.6 mm，5 μm），以0.2%（v/v）甲酸水（A）-乙腈（B）为流动相在360 nm检测波长下梯度洗脱。建立不同产地的16批莲房指纹图谱，并测定莲房药材中6种成分的含量。采用指纹图谱相似度评价和化学计量学分析对不同产地莲房药材进行质量评价。[结果] 16批莲房指纹图谱相似度介于0.984~0.999，共标定了8个共有峰，指认出金丝桃苷、异槲皮苷、山柰酚-3-O-半乳糖苷、异鼠李素3-O-半乳糖苷、异鼠李素-3-O-葡萄糖苷、槲皮素6个成分。聚类分析和主成分分析均可将不同产地的莲房分为两类，偏最小二乘法-判别分析筛选出影响药材质量的3个关键成分异槲皮苷、金丝桃苷和异鼠李素-3-O-半乳糖苷。[结论] 本研究建立的莲房指纹图谱和多成分含量测定方法精密稳定，重复性好，结果可靠，结合化学计量学分析可用于莲房药材的质量评价。

Abstract:

[Objective] To establish the high-performance liquid chromatography chromatographic fingerprint and multi-component content determination method combined with chemometrics analysis，evaluate the quality of Nelumbinis Receptaculum from different regions. [Methods] An Agilent ZORBAX SB-C18 column（250 mm×4.6mm，5 μm） was used for the analysis，with mobile phase 0.2%（v/v） formic acid aqueous solution（A）-acetonitrile（B） gradient elution，and detection wavelength was 360 nm. The fingerprint of 16 batches of Nelumbinis Receptaculum from different regions was established and the content of six main components was determined simultaneously. The fingerprint similarity evaluation and chemometrics analysis were applied to evaluate the quality of Nelumbinis Receptaculum samples from different habitats. [Results] The similarity of fingerprints ranged from 0.984 to 0.999，and eight common peaks were calibrated. Among them，six components were identified，including hyperoside，isoquercetin，kaempferol-3-O-galactoside，isorhamnol-3-O-galactoside，isorhamnol-3-O-glucoside and quercetin. Both cluster analysis and principal component analysis classified 16 batches of Nelumbinis Receptaculum samples from different regions into two categories. Three marker components that affect the quality of the medicinal materials were screeded by orthogonal partial least square discriminate analysis，including isoquercetin，hyperoside and isorhamnol-3-O-galactoside. [Conclusion] The established fingerprint and multi-component content determination method is precise and stable which could be combined with chemometrics analysis to evaluate the quality of Nelumbinis Receptaculum.