| 摘要: |
| [目的] 观察辛通畅络中药复方对血管平滑肌细胞（VSMCs）迁移的影响。[方法] 以含辛通畅络中药处方不同浓度（10%、20%、30%）的大鼠血清培养VSMCs，以正常大鼠空白血清为对照，采用Boyden小室法及划痕实验观察辛通畅络中药血清对VSMCs迁移的影响；采用Western blot检测MMP-2、MMP-9蛋白表达情况。[结果] 辛通畅络中药复方含药血清可以抑制VSMCs的迁移及MMP-2、MMP-9蛋白的表达，并且这种抑制效应呈浓度依赖性。[结论] 辛通畅络中药复方具有抑制VSMCs迁移的作用，其作用机制可能与影响MMP-2和MMP-9的表达有关。 |
| 关键词: 慢性肾脏病 辛通畅络中药复方 细胞迁移 血管平滑肌细胞 |
| DOI：10.11656/j.issn.1672-1519.2017.10.14 |
| 分类号:R285.5 |
| 基金项目:天津市教育委员会课题项目（20100220）。 |
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| Effect of Chinese herbal medicine of Xintong Changluo on the migration of vascular smooth muscle cells in rats |
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YANG Xiao-kun1, MA Xiao-wei2, WANG Meng3, XIAO Xue-feng2
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1.Emergency Department, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;3.The First Hospital of Baoding city, Baoding 071000, China
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| Abstract: |
| [Objective] To observe the effect of Chinese herbal medicine of Xintong Changluo on the migration of vascular smooth muscle cells (VSMCs).[Methods] VSMCs was cultured in serum of rats with different concentrations of Xintong Changluo (10%, 20%, 30%), normal rats' serum was used as control group. The VSMCs migration was observed by scratch test and Boyden chamber method. The expression of MMP-2 and MMP-9 protein was detected by Western blot.[Results] Serum containing Xintong Changluo can inhibit the migration of VSMCs and the expression of MMP-2 and MMP-9 proteins, and the inhibitory effect was concentration dependent.[Conclusions] Xintong Changluo can inhibit the migration of VSMCs, and the mechanism may be related to its influence on the expression of MMP-2 and MMP-9. |
| Key words: chronic kidney disease Chinese herbal medicine of Xintong Changluo migration vascular smooth muscle cell |
