专利方四妙君逸软膏促愈机制的实验研究

Experimental study of mechanism of promoting wound healing of patented product of Simiao Junyi ointment

关键词:  专利方四妙君逸软膏  愈合机制  转化生长因子  表皮生长因子受体  细胞因子

Key words:  patented product of Simiao Junyi ointment  healing mechanism  transforming growth factor  epidermal growth factor receptor  cytokines

摘要:

[目的]研究专利方四妙君逸软膏促愈的作用机制。[方法]将90只大鼠，随机均分为模型组、空白对照组、重组人表皮生长因子组、龙珠软膏组和专利方四妙君逸软膏组5组。建立大鼠皮肤创伤模型后，从宏观整体水平观察不同时相点各组大鼠创面愈合率、组织病理学以及最终创面愈合时间；从微观分子水平观察不同时相点大鼠创面肉芽组织中表皮生长因子受体（EGFR）含量、血清中转化生长因子-α（TGF-α）、白介素-1β（IL-1β）含量。[结果]1）专利方四妙君逸软膏能缩短创面愈合时间（P<0.01），提高创面愈合率。2）专利方四妙君逸软膏能提高大鼠创面EGFR（P<0.01）和血清中TGF-α表达（P<0.01），降低血清中IL-1β的表达（P<0.01），且在第7天时作用最显著。[结论]1）专利方四妙君逸软膏具有良好的促愈作用，且作用与龙珠软膏相当。2）专利方四妙君逸软膏的促愈机制可能与TGF-α、IL-1以及EGFR表达的调节有关。

Abstract:

[Objective] To study the mechanism of promoting wound healing by patented product of Simiao Junyi ointment.[Methods] The 90 rats were randomly divided into model group, blank control group, recombinant human epidermal growth factor group, the Longzhu ointment group and patented product of Simiao Junyi ointment group. Skin wound healing model of rats were successfully established. To observe each group of rats' wound healing time and wound healing rate, histopathology, epidermal growth factor receptor (EGFR), transforming growth factor-alpha(TGF-α), interleukin-1 (IL-1) content.[Results] 1) Patented product of Simiao Junyi ointment can shorten wound healing time (P<0.01) and improve the healing rate. 2) The content of the EGFR in granulation tissue (P<0.01) and TGF-α content in serum (P<0.01) was enhanced. The content of serum IL-1β was reduced (P<0.01).[Conclusion] 1) The patented product of Simiao Junyi ointment has an act of promoting wound healing as the same act of Longzhu ointment. 2) The mechanism of promoting wound healing of patented product of Junyi Simiao ointment may related to the regulation of TGF-α, IL-1 and EGFR.