| 摘要: |
| [目的]研究虫草素对糖毒性胰腺INS-1E细胞保护作用。[方法]建立胰腺INS-1E细胞糖毒性模型，给予虫草素浓度分别为6.125、12.5、25 μmol/L处理，用CCK-8细胞技术试剂盒检测虫草素保护糖毒性胰腺INS-1E细胞存活率，用胰岛素酶联免疫吸附法（ELISA）试剂盒检测虫草素促进INS-1E细胞胰岛素分泌和合成作用，用蛋白免疫印迹（Western-blot）方法检测虫草素促进INS-1E细胞Akt磷酸化作用。[结果]虫草素能够提高糖毒性胰腺INS-1E细胞存活率，促进胰岛素的分泌和合成，提高蛋白激酶Akt的磷酸化作用。[结论]虫草素能对糖毒性胰腺INS-1E细胞具有一定的保护作用。 |
| 关键词: 糖毒性 虫草素 Akt 胰腺 2NS-1E |
| DOI：10.11656/j.issn.1672-1519.2018.02.18 |
| 分类号:R285.5 |
| 基金项目:深圳市南山区科研基金项目（2016014）；广东省中医药强省科研基金项目（20141241）。 |
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| Protection of Cordycepin in pancreatic INS-1E cells against glucotoxicity |
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ZHAO Haiyan, CHEN Libo, LI Liping, HUANG Xing, LI Jing, HU Yuanyuan, LYU Shuxin
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Shenzhen Nanshan District People's Hospital, Shenzhen 518052, China
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| Abstract: |
| [Objective] To study the role of Cordycepin in its protection of pancreatic INS-1E cells against glucotoxicity.[Methods] To establish glucotoxicity INS-1Ecell model, the INS-1E cells viability was observed under the action of different concentrations of Cordycepin (6.125, 12.5, 25 μmol/L) by CCK-8, insulin secretion and synthesis was measured by ELISA Kit, phosphorylation of Akt was detected by Western-blot.[Results] Cordycepin improved viability in glucotoxicity pancreatic INS-1E cells, promoted insulin secretion and synthesis, increased phosphorylation of protein kinase Akt.[Conclusion] Cordycepin have protective effect in glucotoxicity pancreatic INS-1E cells. |
| Key words: glucotoxicity Cordycepin Akt pancreas INS-IE |
