大黄素与丹参素合用对四氯化碳诱导大鼠肝纤维化的保护作用

Effects of combination of emodin and tanshinol in hepatic fibrosis rats induced by carbon tetrachloride

关键词:  大黄素  丹参素  肝纤维化  肝功能  保护作用

Key words:  emodin  tanshinol  hepatic fibrosis  liver function  protective effect

摘要:

[目的]观察大黄素丹参素联合应用对四氯化碳（CCl₄）肝纤维化大鼠的影响。[方法]健康雄性SD大鼠50只，随机分为正常组、模型组、大黄素组、大黄素丹参素合用低剂量组、高剂量组，除空白组外，其余各组均腹腔注射CCl₄建立肝纤维化模型。从第3周开始，除注射造模外，各实验组分别给予相应药物灌胃，正常组、模型组灌胃双蒸水，连续6周，末次给药后，取血和肝脏，观察肝脏的组织病理学改变，检测血中天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）的活性，酶联免疫吸附（ELISA）法测定层粘连蛋白（LN）、透明质酸（HA）和Ⅲ型前胶原（PCⅢ）的含量，酸水解法检测肝组织中羟脯胺酸（HYP）的含量。[结果]与模型组比较，各给药组大鼠血清中ALT、AST、ALP活性和LN、HA、PCⅢ的含量显著降低，肝组织中HYP含量降低（P<0.05，P<0.01），肝组织病理学明显改善；与大黄素组相比，合用高剂量组大鼠ALT、AST、ALP活性和LN、HA的含量降低，肝组织中HYP减少（P<0.05或P<0.01）。[结论]大黄素具有抗肝纤维化的作用，丹参素可增强大黄素抗肝纤维化的效应。

Abstract:

[Objective] To study the effects of the combination of emodin and tanshinol in hepatic fibrosis rats with carbon tetrachloride (CCl₄).[Methods] Fifty male SD rats were randomly divided into normal group, model group, emodin group, high dose emodin and tanshinol combination group, low dose emodin and tanshinol combination group. The hepatic fibrosis rat models in each group were induced by intraperitoneal injection of CCl₄ except normal group. Starting from the third week, in addition to injection molding, each experimental group was given corresponding drug gavage respectively, the normal group and model group were given intragastric administration of physiological saline for 6 weeks. After the last administration, blood and liver tissue were taken to observe the histopathological changes of the liver. The activities of serum alanin aspartate aminotransferase (AST), aminotransferase(ALT) and alkaline phosphatase(ALP) were measured. Levels of laminin (LN), hyaluronic acid (HA) and procollagen Ⅲ(PCⅢ) in serum were detected by enzyme linked immunosorbent assay (ELISA). The content of hydroxyproline (HYP) in liver homogenate was measured by acid hydrolysis method.[Results] Compared with the model group, the levels of ALT, AST, ALP, LN, HA, PCⅢ and HYP liver tissue of each experimental group was significantly decreased(P<0.05, P<0.01), the liver pathological fibrosis was obviously relieve. Compared with emodin group, the levels of ALT, AST, ALP, LN, HA and HYP in high dose emodin and tanshinol combination group were significantly decreased (P<0.05 or P<0.01).[Conclusion] Emodin has antifibrotic effect; tanshinol can enhance the effect of emodin on antifibrotic effect.