超高效液相色谱法同时测定金花葵花中5个黄酮类成分含量

Simultaneous determination of five flavonoids in the flowers of Aurea helianthus by ultra performance liquid chromatography method

关键词:  金花葵花  超高效液相色谱法  黄酮  含量测定

Key words:  flowers of Aurea helianthus  UPLC  flavonoids  quantification

摘要:

[目的]建立超高效液相色谱法（UPLC）同时测定金花葵花提取物中5个黄酮类成分（芦丁、金丝桃苷、异槲皮苷、杨梅素和槲皮素）的含量，以科学评价金花葵花的质量。[方法]采用ACQUITY UPLC BEH C₁₈（1.7 μm，2.1 mm×100 mm）色谱柱，以乙腈和0.1%甲酸水为流动相，流速0.3 mL/min，柱温35℃，检测波长360 nm。[结果]芦丁、金丝桃苷、异槲皮苷、杨梅素、槲皮素分别在0.2~50、2.4~300、0.8~200、0.2~50、0.08~25 μg/mL与峰面积呈现良好的线性关系，r² ≥ 0.999 0，精密度，重复性，稳定性良好，平均加样回收率（n=6）在95%~105%之间。不同批次间金花葵花中5种化合物含量差异较大。[结论]本方法快速，准确，可靠，适用于金花葵花定量分析及质量评价。

Abstract:

[Objective] To evaluate the quality of flowers of Aurea helianthus, ultra performance liquid chromatography(UPLC) method was established and validiated for simultaneous determining 5 flavonoids including rutin, hyperoside, isoquercitrin, myricetin and quercetin in Abelmoschusmanihot flowers.[Methods] The separation of 5 flavonoids was carried out on an ACQUITY UPLC BEH C18 (1.7 μm, 2.1×100 mm) with gradient elutionusing acetonitrile and water containing 0.1% formic acid. The flow rate was 0.3 mL/min. The temperature was maintained at 35℃. The wavelength was recorded at 360 nm.[Results] All five flavonoids within their tested ranges showed good linear relationship with correlation coefficients which are greater than 0.999 0. The results showed good precisions, repeat abilities and stablities and the average recoveries were between 95% and 105%. The contents of five flavonoids existed striking differences among different harvest times and producing areas.[Conclusion] This fast, accurate and reliable method was could be used as tool for quantitative analysis of 5 flavonoids and evaluation of quality of flowers of Aurea helianthus.