| 摘要: |
| [目的] 观察银杏叶提取物EGB761对动脉粥样硬化家兔对氧磷酶-1(PON-1)和氧化低密度脂蛋白(ox-LDL)的影响。[方法] 将24只日本大耳白兔随机分为正常组、模型组、EGB761组(n=8)。正常组给予普通饮食,模型组和EGB761组高脂饮食10周。EGB761组从第7周起给予EGB76150mg/(kg·d)灌胃4周,正常组和模型组给予等量生理盐水。采用酶免疫吸附法(ELISA法)和生化法检测PON-1的活性和含量,ELISA法检测ox-LDL的含量。[结果] 模型组PON-1活性和含量明显降低(P<0.05),ox-LDL的含量明显升高,银杏叶提取物组PON-1活性和含量明显升高,ox-LDL明显降低(P<0.05)。[结论] 银杏叶提取物EGB761降低ox-LDL、抗动脉粥样梗化(AS)的机制可能与其上调PON-1活性和含量有关。 |
| 关键词: 银杏叶提取物EGB761 对氧磷酶-1 氧化低密度脂蛋白 动脉粥样硬化 |
| DOI：10.11656/j.issn.1673-9043.2016.02.07 |
| 分类号: |
| 基金项目:国家自然科学基金项目(830472117,81202797);天津市自然科学基金项目(08JCYBJC10800)。 |
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| Effects of EGB761 on PON-1 and ox-LDL in rabbits with atherosclerosis |
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GUO Mao-juan, SUO Yan-rong, ZENG Wen-yun, YANG Lin, LI Hu-hu, JIANG Xi-juan
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Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To observe effect of EGB761 on PON-1 and ox-LDL in rabbits with atherosclerosis. [Methods] Twenty-four Japanese white rabbits were randomly divided into normal group, model group and EGB761 group. Rabbits in normal group were fed with ordinary diet; model and EGB761 group were given high-fat diet for 10 weeks. Meanwhile, from the seventh week, rabbits in EGB761 group were given 50 mg/(kg·d) of EGB761, control group and model group were given normal saline for 4 weeks. Ten weeks later, the vitality and content of PON-1 were detected by Elisa and biochemical methods, the ox-LDL were detected by Elisa method. [Results] Compared with the model group, the vitality and content of PON1 in model group were higher (P<0.05) and the ox-LDL levels were lower, while in EGB761 group the vitality and content of PON-1 increased significantly and ox-LDL decreased obviously (P<0.05). [Conclusion] The effect of EGB761 on reducing the level of ox-LDL in AS rabbits may response to raise the vitality and content of PON-1. |
| Key words: EGB761 PON-1 ox-LDL AS |
