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| 脑心通胶囊提取物通过增强CXCR4表达促进心脏干细胞迁移 |
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杨岳1, 李慧影1, 张璐莎1, 马亚珂1, 赵步长2, 贾力夫2, 赵菁2, 陈璐1, 王虹1
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1.天津中医药大学中医药研究院, 天津 300193;2.陕西步长集团, 西安 710082
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| 摘要: |
| [目的] 探讨脑心通(NXT)对小鼠心脏干细胞(CSCs)CXCR4表达的影响。[方法] 应用实时荧光定量聚合酶链式反应(PCR)芯片技术研究脑心通对心脏干细胞功能基因表达的影响。采用流式细胞术检测脑心通对心脏干细胞CXCR4表达的影响。应用蛋白免疫印迹法(WesternBlot)检测脑心通对心脏干细胞CXCR4蛋白表达的影响。应用Transwell法测定脑心通对SDF-1诱导的心脏干细胞迁移能力的影响。[结果] 芯片结果发现脑心通促进心脏干细胞CXCR4基因的表达;流式细胞技术结果显示,脑心通促进心脏干细胞APC-CXCR4阳性细胞率;Western Blot结果表明,脑心通促进心脏干细胞CXCR4蛋白表达;Transwell迁移实验结果显示,脑心通促进SDF-1诱导的心脏干细胞的迁移作用。[结论] 脑心通可以促进心脏干细胞CXCR4的表达。 |
| 关键词: 脑心通 心脏干细胞 CXCR4的表达 |
| DOI：10.11656/j.issn.1673-9043.2016.02.08 |
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| 基金项目:国家自然科学基金项目(81173592);新世纪优秀人才支持计划项目(NCET-13-0935);国家国际科技合作专项(2015DFA30430);长江学者和创新团队发展计划(IRT1276)。 |
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| Naoxintong extract promotes migration of cardiac stem cells through enhancing expression of CXCR4 |
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YANG Yue1, LI Hui-ying1, ZHANG Lu-sha1, MA Ya-ke1, ZHAO Bu-chang2, JIA Li-fu2, ZHAO Jing2, CHEN Lu1, WANG Hong1
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1.Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Shanxi Buchang Pharma, Xi'an 710082, China
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| Abstract: |
| [Objective] To study the effect of Naoxintong(NXT) on cardiacstem cell function and to explore its possible mechanism.[Methods] We use real-time RT-PCR array technology, Western-blot ,flow cytometry and transwell methods to investigate the expression of CXCR4 on the cardiac stem/progenitor cell by NXT. [Results] The results showed that the expression of CXCR4 gene and CXCR4 protein in cardiac stem cell were both increased by NXT. [Conclusion] The migration of cardiac stem cell is promoted by NXT through the SDF-1/CXCR4 axis. |
| Key words: NXT cardiac stem cells expression of CXCR4 |
