当归补血汤对糖尿病大鼠肾组织NF-κB、MCP-1表达的影响

Effect of Astragals and Angelica Mixture on renal expression of NF-κB and MCP-1 in rats with diabetic

关键词:  当归补血汤  糖尿病肾病  炎症  核因子-κB  单核细胞趋化蛋白-1

Key words:  astragals and angelica mixture  diabetic nephropathy  inflammation  NF-κB  MCP-1

摘要:

[目的] 观察当归补血汤干预下糖尿病大鼠肾脏核因子-κB（NF-κB）、单核细胞趋化蛋白-1（MCP-1）炎症因子表达变化，探讨当归补血汤抗炎机制及其与肾脏保护作用的相关性。[方法] 链脲佐菌素（STZ）+高脂饲料建立糖尿病肾病（DN）动物模型，酶偶联比色法检测血糖、血脂及肾功能相关指标，光镜、电镜下观察肾脏结构变化，酶联免疫吸附试验检测大鼠肾脏NF-κB、MCP-1蛋白含量，实时荧光定量聚合酶链式反应（RT-PCR）检测肾脏NF-κBp65、MCP-1 mRNA表达变化。[结果] DN大鼠肾功能下降，肾脏损伤严重，肾组织NF-κB、MCP-1蛋白含量增加，NF-κBp65、MCP-1mRNA转录活性增强，当归补血汤治疗后肾功能明显改善，肾脏NF-κB、MCP-1蛋白含量降低，NF-κBp65、MCP-1 mRNA转录活性下降。[结论] 当归补血汤可有效减少DN大鼠肾脏NF-κB、MCP-1的表达及活性，抑制肾脏炎症反应，减轻肾脏损伤。

Abstract:

[Objective] To investigate the anti-inflammation mechanism of Astragals and Angelica Mixture with an influence on the expression of inflammatory factors NF-κB and MCP-1, and its correlation with renal protective effect. [Methods] Using STZ+high-fat diet to establish animal models of DN. Enzyme coupling colorimetric method to detect the serum glucose, lipids and the renal function related parameters. Kidney structure change was observed under the light and electron microscope, Enzyme-linked immune sorbent assay to detect the protein content of NF-κB and MCP-1 in the kidney. Real-time fluorescent quantitative PCR to measure the change of NF-κB and MCP-1 mRNA expression. [Results] The protein and mRNA expression of NF-κB and MCP-1 in DN rats kidney was significantly increased. After treated with Astragalus and Angelica Mixture, the renal function was improved, kidney protein and mRNA expression of NF-κB, MCP-1 was significantly reduced. [Conclusion] Astragals and Angelica Mixture can effectively decrease the protein and mRNA expression and activity of NF-κB and MCP-1 in DN rats kidney, ameliorate kidney damage, have a anti-inflammation effect.