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| UPLC法同时测定地锦草中4种指标成分的含量 |
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冯光维1,2, 刘志东1, 祁东利1, 皮佳鑫1, 顾星1, 张谦1, 黄瑞1
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1.天津中医药大学 现代中药发现与制剂技术教育部工程研究中心, 天津 300193;2.黔南民族医学高等专科学校, 都匀 558000
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| 摘要: |
| [目的] 建立同时测定地锦草药材中没食子酸、鞣花酸、槲皮素、山奈酚4种指标成分的超高液相色谱法（UPLC）。[方法] 采用UPLC法，色谱柱为Acquity UPLC BEH C18柱（100 mm×2.1 mm，1.7 μm）；流动相为甲醇-乙腈4∶6（A）-0.2%甲酸水（B）溶液，梯度洗脱：0～3 min，5% A；3～4 min，5%～17% A；4～19 min，17% A；19～42 min，17%～30% A；42～50 min，30%～34% A；50～55 min，34%～70% A，体积流量0.2 mL/min；检测波长为0～4 min, 270 nm；4～55 min，360 nm，柱温30℃。[结果] 4种成分均达到基线分离，各成分在线性范围内有良好的线性关系（r>0.999 7）；回收率在 96.5%～102.9%。[结论] 建立的测定方法准确灵敏、重复性好，能较全面地评价地锦草药材的质量。 |
| 关键词: UPLC 同时测定 地锦草 含量 |
| DOI：10.11656/j.issn.1673-9043.2016.03.12 |
| 分类号: |
| 基金项目:黔南民族医学高等专科学校科研基金资助项目（QNYZ2014014）。 |
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| Simultaneous determination of four components in Euphorbia Humifusa Willd. by UPLC |
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FENG Guang-wei1,2, LIU Zhi-dong1, QI Dong-li1, PI Jia-xin1, GU Xing1, ZHANG Qian1, HUANG Rui1
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1.Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Qiannan Medical College for Nationalities, Duyun, Guizhou, 558000, China
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| Abstract: |
| [Objective] To establish an UPLC method for simultaneous determination four index components such as gallic acid, ellagic acid, quercetin and kaempferol in Euphorbia Humifusa Willd. [Methods] A chromatographic separation method was established with Acquity UPLC BEH C18 column (100 mm×2.1 mm, 1.7μm) with methanol-acetonitrile (40:60, A)-0.2% formic acid (B) as mobile phases at the flow rate of 0.2 mL/min. The gradient elution was carried out as follows: 0~3 min, 5%A; 3~4 min, 5%~17%A;4~19min, 17%A;19~42 min, 17%~30%A; 42~50 min, 30%~34%A; 50~55 min, 34%~70%A. The detection wavelength was 270 nm at 0~4 min and 360 nm at 4~55 min, and the column temperature was 30℃. [Results] The four index components were well separated and each component had a good linear relationship in the liner range (r>0.999 7); and the recovery was in the range of 96.5%~102.9%. [Conclusion] The method is accurate, sensitive and repeatable for the comprehensive quality control of Euphorbia Humifusa Willd. |
| Key words: UPLC simultaneous determination Euphorbia Humifusa Willd. content |
