丹枝饮对盆腔炎性疾病后遗症小鼠体内炎性因子的影响

Effect of Danzhi decoction on serum levels of inflammatory factors in murine model with sequelae of pelvic inflammatory disease

关键词:  盆腔炎性疾病后遗症  丹枝饮  炎性因子

Key words:  Sequelae of pelvic inflammatory disease  Danzhi Decoction  inflammatory factor

摘要:

[目的] 观察丹枝饮对盆腔炎性疾病后遗症（SPID）小鼠血清炎性因子白介素-17A（IL-17A）、白介素-17F（IL-17F）、单核细胞趋化蛋白-1（MCP-1）、巨噬细胞炎症蛋白-2（MIP-2）表达水平的影响，探讨盆腔炎性疾病后遗症的发病机制及丹枝饮治疗SPID的药理作用机制。[方法] 将48只SPF级BALB/c小鼠随机分为正常组、模型组、阿司匹林组[20.55 mg/（kg·d）]、丹枝饮小剂量组[7.91 g/（kg·d）]、丹枝饮中剂量组[15.82 g/（kg·d）]、丹枝饮大剂量组[31.64 g/（kg·d）]，每组8只。其中模型组、阿司匹林组、丹枝饮小、中、大剂量组采用宫腔种植鼠衣原体复制SPID小鼠模型，造模后第6周开始灌胃给药3个动情周期（15 d）后，检测各组小鼠脸谱疼痛评分（MGS），血清IL-17A、IL-17F、MCP-1、MIP-2的表达水平。[结果] 与模型组相比较，阿司匹林组、丹枝饮小、中、大剂量组MGS评分，血清IL-17A、IL-17F、MCP-1、MIP-2的表达水平均有显著降低（P<0.01），且丹枝饮中剂量组在各项指标的降低程度上均优于阿司匹林组、丹枝饮小剂量组及丹枝饮大剂量组（P<0.05）。[结论] 盆腔炎性疾病后遗症可以引起炎性因子IL-17A、IL-17F、MCP-1、MIP-2的表达水平的增高，丹枝饮可以有效的减轻SPID小鼠脸谱疼痛评分，降低SPID小鼠血清炎性因子IL-17A、IL-17F、MCP-1、MIP-2的表达水平，从而达到治疗SPID的作用。

Abstract:

[Objective] To investigate the effect of Danzhi Dection (DZD) on serum levels of interleukin-17A(IL-17A), interleukin-17F(IL-17F), monocyte chemoattractant protein-1(MCP-1) and macrophage inflammatory protein-2(MIP-2) in murine model with sequelae of pelvic inflammatory disease. To explore the pathogenesis of sequelae of pelvic inflammatory disease and explain the pharmacological action of Danzhi Dectionin treating SPID.[Methods] 48 BALB/c mice were randomly divided into normal control group, model group, aspirin (ASP) group[20.55 mg/(kg·d)], DZD low-dose group[7.91 mg/(kg·d)], DZD mid-dose group[15.82 mg/(kg·d)] and DZD high-dose group[31.64 mg/(kg·d)], 8 mice in each group. The mice of the model group, ASP group, DZD low-dose group, DZD mid-dose group and DZD high-dose group were given injected Chlamydia muridarum into the uterine cavity to induce SPID. After modeling of 6 weeks, each group were given medicine for 15 d. The mouse grimace scale(MGS), the serum levels of IL-17A, IL-17F, MCP-1 and MIP-2 of mice were detected.[Results] To compare with model group, the MGS, the serum levels of IL-17A, IL-17F, MCP-1 and MIP-2 were significantly decreased in ASP group, DZD low-dose group, DZD mid-dose group and DZD high-dose group(P<0.01). And the effect of DZD mid-dose group was better than other treatment groups(P<0.05).[Conclusion] SPID can enhance the expression levels of IL-17A, IL-17F, MCP-1 and MIP-2, Danzhi Dection can effective reduce the MGS, the serum levels of IL-17A, IL-17F, MCP-1 and MIP-2 in SPID mice. Danzhi Dection is the better choice to treat SPID.