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| HPLC法同时测定达原饮中芒果苷等6种成分的含量 |
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王金艳1,2, 任静1,2, 陈世彬3, 姚少姿1,2, 马丽1,2, 刘志东1,2
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1.天津中医药大学, 现代中药发现与制剂技术教育部工程中心, 天津 300193;2.天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;3.华润三九医药股份有限公司, 深圳 518110
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| 摘要: |
| [目的]探讨高效液相色谱法（HPLC）建立同时测定达原饮中芒果苷、芍药苷、黄芩苷、甘草酸、厚朴酚、和厚朴酚6种活性成分的含量测定方法。[方法]采用Agilent Zorbax Eclipse Plus C18（4.6 mm×250 mm，5 μm）色谱柱，流动相为乙腈（A）-0.2%磷酸水（B）。梯度洗脱：0~40 min，10%~40% A；40~45 min，40%~70% A；45~50 min，70% A；50~55 min，70%~10% A；55~60 min，10% A。流速0.8 mL/min，柱温30℃，检测波长230 nm。[结果] 6种成分分离度良好，各成分在线性范围内有良好的线性关系；回收率（n=9）在95.0%~103.0范围内，RSD%值均小于3.0%。[结论]该方法简便，准确度高，灵敏度、重复性好，可用于达原饮的质量控制。 |
| 关键词: 达原饮 含量测定 芒果苷 芍药苷 黄芩苷 |
| DOI：10.11656/j.issn.1673-9043.2018.01.17 |
| 分类号:R285.5 |
| 基金项目: |
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| Simultaneous determination of six components such as mangiferin in Dayuan yin by HPLC |
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WANG Jinyan1,2, REN Jing1,2, CHEN Shibin3, YAO Shaozi1,2, MA Li1,2, LIU Zhidong1,2
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1.Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2.Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China;3.China Resources of Sanjiu Medical & Pharmaceutical CO., LTD., Shenzhen 518110, China
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| Abstract: |
| [Objective] Use HPLC to establish a method which can determine 6 kinds of index component in Dayuan yin, such as mangiferin, paeoniflorin, baicalin, glycyrrhizic acid, magnolol and honokiol.[Methods] The Agilent Zorbax Eclipse Plus C18 (4.6 mm×250 mm, 5 μm) column was adopted; the mobile phase was acetonitrile (A)-0.2% aqueous phosphoric acid (B) by a gradient eluted program:0~40 min, 10%~40% A; 40~45 min, 40%~70% A; 45~50 min, 70% A; 50~55 min, 70%~10% A; 55~60 min, 10% A. The flow rate was 0.8 mL/min, The column temperature was 30℃, The detection wavelength was 230 nm.[Results] The separation degree of the 6 components was good, and all the components had good linear relationship in the linear range, and the recoveries (n=9) were within the range of 95.0%~103.0%, the RSD% were less than 3.0%.[Conclusion] The method is simple, accurate, sensitive and reproducible, and can used to determine the contents of Dayuan yin. |
| Key words: Dayuan yin determination of contents mangiferin paeoniflorin baicalin |
