清肝通络饮对SHR的降压及心脏保护作用的研究

Antihypertensive effects of Qinggan Tongluo yin in spontaneously hypertensive rats and protective effect on SHR heart

关键词:  清肝通络饮  天麻钩藤饮  原发性高血压  徐宗佩教授

Key words:  Qinggan Tongluo yin  Tianma Gouteng decoction  essential hypertension  professor XU Zongpei

摘要:

[目的] 清肝通络饮是天津中医药大学徐宗佩教授临床经验方，本实验观察其对自发性高血压大鼠（SHR）血压、心脏功能及形态学的影响，同时观察清肝通络饮对SHR的血脂的影响。[方法] 应用SHR模型，观察单次灌服清肝通络饮后1、3、4、6 h时及连续灌胃给予清肝通络饮10周，大鼠SBP的变化；应用小动物超声测量仪检测给药10周后，大鼠左室射血分数、左室短轴缩短率、左室内径、室间隔厚度等反映左心室功能和形态结构的指标；检测血脂生化指标，研究清肝通络饮对血脂的调节作用。[结果] 单次灌胃清肝通络饮干预后，未能显著降低SHR血压；连续10周给药后，清肝通络饮[25 g生药/（kg·d）]组在第1周、第5周能够降低SHR收缩压（P<0.05）；清肝通络饮[6.25 g生药/（kg·d）]组在第1周、第7周、第8周能降低SHR收缩压（P<0.05）；阳性对照药缬沙坦单次给药及连续10周多次给药，均能降低SHR血压（P<0.05）；阳性对照药组、各组清肝通络饮组均能显著增加心脏射血分数和左室短轴缩短率（P<0.05），能显著降低左室收缩末期内径（P<0.05）、左室收缩末期容积（P<0.05），增加收缩末期室间隔厚度（P<0.01）；未观察到清肝通络饮对SHR大鼠血脂有显著影响。[结论] 各组清肝通络饮能改善自发性高血压大鼠心脏形态，提高心脏功能，减轻左心室肥厚。

Abstract:

[Objective] Qinggan Tongluo yin(QGTLY) is a clinical experience based on professor XU Zongpei. To study the effects of QGTLY to spontaneously hypertensive rats (SHR) blood pressure, cardiac function and morphology, blood lipid.[Methods] By using SHR,measure blood pressure SBP on 1, 3, 4, 6 hours after the rat was tread with QGTLY one time, measure blood pressure SBP on 10 weeks after the rat was tread with QGTLY continuously. The indicators of left ventricular function and morphological structure were measured by small animal ultrasonic measuring instrument, such as LVEF, LVFS, Lvid, Ivs etc. Observe the changes of treated with QGTLY continuously on 10 weeks to study the effects on lipid.[Results] Treating with different dose group of QGTLY one time can not reduce the SBP of SHR. Treating with different dose group of QGTLY continuously 10 weeks. Treating with QGTLY[25 g/(kg·d)] redue the SBP of SHR at 1weeks, 5 weeks(P<0.05~0.01). Treating with QGTLY[6.25g/(kg·d)] reduce the SBP of SHR at 1 weeks, 7 weeks, 8 weeks(P<0.05). Treating with Valsartan one time or continuously 10 weeks can reduce the blood SBP(P<0.05~0.01). Different dose group of QGTLY can improve the EF, FS(P<0.05~0.01), reduce the left ventricular systole(P<0.05~0.01) and the end volume of the left ventricle(P<0.05~0.01), increase the interventricular septum thickness(P<0.01). There were no changes in lipids.[Conclusion] QGTLY can improve cardiac morphology, increase cardiac function, and reduce left ventricular hypertrophy on SHR.