麻黄饮片等级质量标准研究

Study on the grade quality standard of Ephedra Sinica

关键词:  麻黄  等级质量评价  化学计量学  HPLC

Key words:  Ephedra  grade quality evaluation  stoichiometrically science  HPLC

摘要:

[目的] 以31批麻黄饮片为研究对象，采用高效液相色谱（HPLC）结合化学计量学的方法初步建立麻黄饮片的等级质量标准。[方法] 依照《中华人民共和国药典》对31批次麻黄饮片的浸出物、灰分、水分进行检测；采用HPLC测定31批麻黄饮片中麻黄碱和伪麻黄碱的含量；通过建立主成分分析（PCA）、聚类分析（CA）和偏最小二判别分析（PLS-DA）多种化学模式识别模型对麻黄进行等级分类研究。[结果] 31批麻黄饮片的水溶性浸出物含量在13.05%~28.11%；总灰分为5.84%~8.94%；平均含水量为3.75%。麻黄碱及伪麻黄碱含量为1.21%~3.35%。[结论] 该实验初步将麻黄饮片分为优等、统货两个等级，为其他中药饮片数据化和标准化研究提供新思路。

Abstract:

[Objective] Study on 31 batches of Ephedra Sinca.The qulity standard of Ephedra Sinica tablets was established by High-performance liquid chromatography(HPLC) combined with chemometrics method. [Methods] According to the Chinese Pharmacopoeia, the extracts, ash and moisture of 31 batches of Ephedra Sinica Yinpian weretested;determination of ephedrine and pseudoephedrine in 31 batches of Ephedra Sinica Yinpianuuui;grade classification study on Ephedra Sinica by establishing chemical composition identification model(PCA), cluster analysis(CA) and partial least two discriminant analysis(PIS-DA). [Results] The moisture diffuse content of 31 batches of Ephedra tablets was 13.05% -28.11%;the total ash was 5.84% -8.94%;the average water content was 3.75%. The content of ephedrine and pseudoephedrine was 1.21%-3.35%. [Conclusion] This experiment initially divided the Mahuang decoction into two grades, namely, superior and unified goods.Chinese Herbal Medicine and other standardized data WH study provides a new idea.