芍药-甘草不同配伍比例提取物中有效成分溶出规律的研究

Study on the dissolution rules and quality markers of active components in the extracts of paeonia-liquorice with different compatibility ratios

关键词:  芍药-甘草  配伍比例  有效成分溶出率

Key words:  shaoyao-gancao  compatibility ratio  active ingredient dissolution rate

摘要:

[目的]通过测定不同比例芍药-甘草药对提取液的pH值、密度、出膏率和6种有效成分的含量综合探讨相关指标随芍药-甘草药对配伍变化规律，探究芍药-甘草药对的质量标志物。[方法]分别测定出膏率、pH值、密度；采用Kromasil 100-5-C₁₈色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈（B）-0.1%磷酸水溶液（A），梯度洗脱（0～8 min，14%～19% B；8～20 min，19%～28% B；20～25 min，28%～35% B；25～30 min，35%～45% B；30～40 min，45%～95% B，流速1.0 mL/min，检测波长为254 nm，柱温30℃。[结果]芍药-甘草不同比例（1∶0、10∶1、10∶2、10∶3、10∶4、10∶5、10∶6、10∶7、10∶8、1∶1、1∶2、1∶3、1∶4、1∶6、1∶8、0∶1）提取液的密度和pH值与比例大小没有明显关系，密度变化范围为1.003±0.002，pH密度变化范围为4.94±0.11，出膏率变化范围为15.39±1.70。芍药-甘草不同比例组煎煮过程中，芍药中芍药内酯苷和芍药苷的变化趋势较为接近，甘草中甘草苷和甘草酸的变化趋势相近。[结论]综合不同比例芍药-甘草药对提取液的pH值、密度、出膏率和6种有效成分的含量结果，从化学成分层面上，选择芍药内酯苷、芍药苷、甘草苷和甘草酸作为芍药-甘草药对的质量标志物科学合理。

Abstract:

[Objective] By measuring the ratio of pH value,density,ointment rate,polysaccharide content and content of six active ingredients in different proportions of shaoyao-gancao decoction,the related indexes were studied with the variation of the compatibility change law,and exploring the quality markers of shaoyao-gancao pairs.[Methods] To determinate the paste rate,the ratio of pH value and density,separately. The determination was carried out with Kromasil 100-5-C₁₈ column (250 mm×4.6 mm,5 μm). The mobile phase consisted of acetonitrile (B)-0.1% phosphoric acid aqueous solution (A) with gradient elution manner (0~8 min,14%~19%B,8~20 min, 19%~28% B,20~25 min,28%~35% B,25~30 min,35%~45% B,30~40 min,45%~95% B). The flow rate was 1.0 mL/min,the detection wavelength was 254 nm and the column temperature was 30℃.[Results] There was no significant relationship between the density and the pH value of the extract and the ratio (1:0,10:1,10:2,10:3,10:4, 10:5,10:6,10:7,10:8,1:1,1:2,1:3,1:4,1:6,1:8,0:1). The range of density change was 1.003±0.002,the range of pH change was 4.94±0.11,and the range of paste rate change was 15.39±1.70. In the decoction process of different proportions of shaoyao-gancao,the change trend of albiflorin and paeoniflorin in shaoyao was similar,and the change trend of liquiritin and glycyrrhizic acid in gancao was similar.[Conclusion] According to the results of different ratios of shaoyao-gancao extract pH,density,paste rate and content of six active ingredients. From the chemical composition level,it is scientific and reasonable to choose albiflorin,paeoniflorin,liquiritin and glycyrrhizic acid as the quality marker of shaoyao-gancao.