| 摘要: |
| [目的] 通过建立中药细辛水提物高效液相色谱（HPLC）指纹图谱，评价不同产地细辛药材水提物的质量差异。[方法] 采用ZORBAX Eclipse Plus C18柱（4.6 mm×250 mm，5 μm）；以0.1%甲酸水溶液（A）-乙腈（B）为流动相进行梯度洗脱洗；流速为1 mL/min；柱温：30 ℃；进样量：5 μL；检测波长为285 nm。建立10批细辛药材水提物的指纹图谱，通过相似度分析并结合聚类分析（HCA）、偏最小二乘判别分析（PLS-DA）、正交偏最小二乘法-判别分析（OPLS-DA）及主成分分析（PCA）对细辛药材水提物进行质量评价。[结果] 建立的细辛药材水提物指纹图谱共标定了20个共有峰，通过与对照品比对，指认出6个化学成分，10批样品的相似度均在0.908以上。HCA、PLS-DA、OPLS-DA及PCA结果表明10批细辛药材水提物之间存在一定的质量差异，这些结果可用于对不同产地的细辛药材水提物进行质量评价。[结论] HPLC指纹图谱结合化学模式识别技术可系统、全面地评价细辛药材水提物的质量。对中药材细辛水提物进行研究可进一步完善对细辛药材的质量评价。 |
| 关键词: 细辛 HPLC 指纹图谱 聚类分析 主成分分析 |
| DOI：10.11656/j.issn.1673-9043.2022.02.24 |
| 分类号:R284.2 |
| 基金项目:天津市科技计划项目（20ZYJDJC00120）。 |
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| Study on HPLC fingerprint and chemical pattern recognition of Asarum water extract |
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XUE Zixiang1, WANG Songrui1, YANG Dongyue1, ZHAO Lulu1, OUYANG Huizi2, CHANG Yanxu1, HE Jun1
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1.Tianjin University of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China;2.First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To evaluate the quality difference of water extract of Asarum from different areas by establishing HPLC fingerprint. [Methods] Zorbax eclipse plus C18 column (4.6 mm×250 mm,5 μm) was used;0.1% formic acid aqueous solution (A)-acetonitrile (B) was used as mobile phase for gradient elution;flow rate was 1 mL/min;column temperature was 30℃;injection volume was 5 μL;detection wavelength was 285 nm. The fingerprints of 10 batches of water extracts of Asarum were established. The quality of water extracts of Asarum was evaluated by similarity analysis,cluster analysis (HCA),partial least squares discriminant analysis (PLS-DA),orthogonal partial least squares discriminant analysis (OPLS-DA) and principal component analysis (PCA). [Results] The 20 common peaks were identified in the fingerprint of water extract of Asarum. Six chemical components were identified by comparison with the reference substance. The similarity of 10 batches of samples was more than 0.908. HCA,PLS-DA,OPLS-DA and PCA showed that there were some quality differences among 10 batches of water extracts of Asarum. These results can be used to evaluate the quality of water extracts of Asarum from different areas. [Conclusion] HPLC fingerprint combined with chemical pattern recognition technology can systematically and comprehensively evaluate the quality of water extract of Asarum. The study on the water extract of Asarum can further improve the quality evaluation of Asarum. |
| Key words: Asarum HPLC fingerprint HCA PCA |
