糖脂清提取物对淀粉水解中α-糖苷酶抑制作用研究

Study on the inhibitory effect of Tangzhiqing extract on α-glucosidase in starch hydrolysis

关键词:  淀粉水解  α-糖苷酶抑制  糖脂清提取物  降糖作用

Key words:  tarch hydrolysis  α-glycosidase inhibition  Tangzhiqing extract  hypoglycemic effect

摘要:

[目的] 针对淀粉水解的各个步骤，研究糖脂清提取物（TZQ）对参与淀粉水解的α-糖苷酶的抑制作用，考察TZQ干预淀粉水解的途径。[方法] 以参与淀粉水解各环节的碳水化合物淀粉、糊精、麦芽糖、异麦芽糖为底物，运用体外酶抑制动力学研究的方法，考察TZQ对参与淀粉逐级水解的α-糖苷酶（淀粉酶、糊精酶、麦芽糖酶和异麦芽糖酶）的抑制作用。[结果] 在6.88×10^-5～5.50×10^-4 mg/mL浓度范围内，TZQ对淀粉酶活性的抑制作用未显示剂量依赖性；在1.10×10^-4～6.60×10^-3 mg/mL浓度范围内，TZQ对糊精酶活性没有明显的抑制作用；在5.50×10^-4～1.10×10^-2 mg/mL浓度范围内，TZQ对麦芽糖酶活性具有较好的抑制作用，IC₅₀值为2.96×10^-3 mg/mL；在5.50×10^-4～1.10×10^-2 mg/mL浓度范围内，TZQ对异麦芽糖酶活性具有较好的抑制作用，IC₅₀值为1.77×10^-3 mg/mL。[结论] TZQ可通过抑制淀粉酶、麦芽糖酶、异麦芽糖酶活性干预淀粉水解过程降低血糖，TZQ为一潜在的α-糖苷酶抑制剂。

Abstract:

[Objective] To investigate the inhibitory effect of Tangzhiqing(TZQ) on α-glucosidase involved in starch hydrolysis and the pathway of TZQ intervention in starch hydrolysis,focusing on each step of starch hydrolysis. [Methods] Using carbohydrates such as starch,dextrin,maltose,and isomaltose as substrates,the inhibitory effect of TZQ on α-glucosidase amylase,dextrinase,maltase,and isomaltase involved in starch hydrolysis was investigated using in vitro enzyme inhibition kinetics. [Results] Within the concentration range of 6.88×10^-5 to 5.50×10^-4 mg/mL,TZQ showed no dose-dependent inhibition of amylase activity;within the concentration range of 1.10×10^-4 to 6.60×10^-3 mg/mL,TZQ showed no significant inhibition of dextrinase activity;within the concentration range of 5.50×10^-4 mg/mL to 1.10×10^-2 mg/mL,TZQ showed a good inhibitory effect on maltase activity with an IC₅₀ value of 2.96×10^-3 mg/mL;within the concentration range of 5.50×10^-4 mg/mL to 1.10×10^-2 mg/mL,TZQ showed a good inhibitory effect on isomaltase activity with an IC₅₀ value of 1.77×10^-3 mg/mL. [Conclusion] TZQ can interfere with starch hydrolysis by inhibiting the activity of amylase,maltase,and isomaltase,thereby reducing blood glucose levels. TZQ is a potential α-glucosidase inhibitor.